中国中药杂志

2020, v.45(21) 5160-5168

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自然林和人工搭棚种植模式下黄连根际土壤真菌群落结构变化
Variation in fungal community structures in rhizosphere soil of Coptis chinensis with cropping mode under natural forest and artificial shed

王钰;谭均;伍晓丽;莫让瑜;陈大霞;
WANG Yu;TAN Jun;WU Xiao-li;MO Rang-yu;CHEN Da-xia;Chongqing Sub-center of National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences,Chongqing Key Laboratory of Chinese Medicine Resources, Institute of Material Medical Planting, Chongqing Academy of Chinese Materia Medica;

摘要(Abstract):

采用高通量测序技术对自然林和人工搭棚种植模式下一至五年生黄连根际土壤中的真菌群落进行了测定,分析了土壤真菌的Alpha多样性、群落组成和群落结构差异,并研究了土壤理化性质对真菌群落结构的影响。旨在从根际土壤真菌群落特征诠释2种种植模式下黄连根际土壤微生态的差异,为深入了解黄连土壤健康状况及黄连生态种植提供科学依据和理论指导。研究结果表明:在2种种植模式下,二至五年生黄连根际土壤真菌Chao1指数和ACE指数无显著性差异,但均较一年生黄连根际土壤显著升高;对土壤样品进行比较发现,2种种植模式下,一至五年生黄连根际土壤的OTUs数均无显著差异。不同种植模式以及不同种植年限的真菌群落组成和相对丰度存在差异,自然林种植模式下共计检测到真菌17个门、59个纲、155个目,人工搭棚种植模式下共计检测到真菌17个门、59个纲、157个目;子囊菌门Ascomycota、担子菌门Basidiomycota和被孢霉门Mortierellomycota是2种种植模式下的真菌优势种群,3个门的相对丰度之和分别占真菌总量的74.36%,74.30%。群落结构差异检验结果表明:不同种植模式下,同一年生黄连根际土壤相比,一年生、二年生黄连根际土壤真菌群落结构在2种种植模式下存在显著差异,三至五年生黄连根际土壤真菌菌群结构无显著差异。自然林种植模式下,不同年生黄连根际土壤样品间均存在显著差异。人工搭棚模式下,一年生与三至五年生黄连之间,二年生与三至五年生黄连之间土壤根际真菌群落结构呈显著差异。细菌群落结构与土壤理化因子的CCA及相关性分析结果表明,土壤pH和有机质含量对真菌群落结构变化有显著性影响。土壤OM与担子菌门和隐真菌门呈极显著正相关,pH与担子菌门呈显著负相关,与隐真菌门呈极显著负相关。
This study is aimed to reveal the rhizosphere soil fungal community structure difference of Coptis chinensis cropping between natural forest and artificial shed modes, and provide theoretical guidance for soil improvement and C. chinensis planting. The rhizosphere soil samples of 1-5-year-old C. chinensis under natural forest and artificial shed modes were collected. Illumina high-throughput sequencing technology was used to analyze the community structure and diversity of soil fungi under the tow cropping modes,and the effects of soil nutriment indices on soil fungal community structure. The results suggested that the abundance and diversity of fungal communities in soil of 2-5-year-old C. chinensis were not significant different in both two cropping modes, but it was significantly higher than that in the 1-year-old C. chinensis. Comparing soil samples from the same year-old C. chinensis under the two cropping modes, it was found that there was no significant difference in the abundance and diversity of fungal communities. The fungal community of the rhizosphere soil was different in composition and abundance between tow cropping modes, and between different planting years. The 17 phyla,59 classes and 155 orders,and 17 phyla,59 classes and 157 orders were detected in the rhizosphere soil of C. chinensis under the cropping modes of natural forest and the artificial shed, respectively. Ascomycota, Basidiomycota and Mortierellomycota were dominant phyla in rhizosphere soil, and the average abundance of the 3 phyla accounted for 74.36% and 74.30% of the total fungi. The results of analysis of similarities showed that there were significant differences in the fungal community structure of 1-year-old and 2-year-old C. chinensis soil fungi, and there was no significant difference in the community structure of 3-5-year-old samples. Under the natural forest cropping mode, there were significant differences among the samples of different years. Under the artificial shed cropping, there were significant differences in fungal community structure between 1-year-old and 3-5-year-old C. chinensis soil, and between 2-year-old and 3-5-year-old C. chinensis soil. The results of canonical correlation analysis showed that soil pH and soil organic matter content were the main factors affecting the soil fungal community structure. Soil organic matter content was positively correlated with Basidiomycota and Cryptomycota, pH was negatively correlated with Basidiomycota and C. ryptomycota. The planting of C. chinensis has promoted the diversity and abundance of rhizosphere fungal community significantly. For the same year-old C. chinensis soil, abundance of fungal community was no significant difference between two cropping modes. There are significant differences in the rhizosphere soil fungal community structure between tow cropping modes in the first two years of planting. Through the interaction between the rhizosphere and the soil and the continuous selection of the rhizosphere to the fungal community, the fungal community structure tended to be the same between the two cropping modes in rhizosphere soil of 3-5-year old C. chinensis. The soil pH and orga-nic matter content were the main factors affecting the change of fungal community structure.

关键词(KeyWords): 黄连;种植模式;真菌;群落结构
Coptis chinensis;cropping mode;fungi;community structure

Abstract:

Keywords:

基金项目(Foundation): 国家重点研发计划项目(2017YFC1702601);; 重庆市中药研究院基本科研业务费项目(jbky20190022);; 重庆市科卫联合中医科技项目(ZY201802040)

作者(Author): 王钰;谭均;伍晓丽;莫让瑜;陈大霞;
WANG Yu;TAN Jun;WU Xiao-li;MO Rang-yu;CHEN Da-xia;Chongqing Sub-center of National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences,Chongqing Key Laboratory of Chinese Medicine Resources, Institute of Material Medical Planting, Chongqing Academy of Chinese Materia Medica;

Email:

DOI: 10.19540/j.cnki.cjcmm.20200814.101

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