中国中药杂志

2011, v.36(22) 3119-3124

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板蓝根超微粉体二元指纹图谱研究
Study on double fingerprints of Isatidis Radix micropower

冯小燕;张水寒;蔡光先;黄一科;邵怡;
FENG Xiaoyan1,3,ZHANG Shuihan2,3,CAI Guangxian2,3,HUANG Yike1,3,SHAO Yi1,3(1.Hunan University of Chinese Medicine,Changsha 410013,China; 2.Hunan Academy of Chinese Medicine,Changsha 410013,China; 3.Hunan Innovation Team of Teachs hall Chinese Medicine Powder Technology,Hunan Key Laboratory of Chinese Medicine Powder and Innovation Drug Province Department Co-construction,Changsha 410013,China)

摘要(Abstract):

目的:建立13批板蓝根超微粉体水溶性成分和氨基酸柱前衍生化双重HPLC指纹图谱。方法:采用RP-HPLC梯度洗脱,选择Hypersil BDS C18色谱柱(4.6 mm×250 mm,5μm);水溶性成分HPLC指纹图谱的流动相乙腈-0.1%磷酸水溶液,流速0.5 mL.min-1,柱温20℃,进样量10μL;氨基酸成分HPLC指纹图谱样品经异硫氰酸苯酯试剂柱前衍生化处理后进样,流动相0.1 mol.L-1醋酸钠缓冲溶液(pH 6.5)-乙腈,流速1.0 mL.min-1,柱温30℃,进样量5μL;检测波长均为254nm。采用国家药典委员会"中药色谱指纹图谱相似度评价系统(2.0版)"处理分析,建立板蓝根指纹图谱,同时测定了4种主要成分含量,并进行SPSS系统聚类分析。结果:建立了板蓝根超微粉体水溶性成分和氨基酸成分指纹图谱共有模式,确认了腺苷、表告依春和15种氨基酸的特征峰。聚类分析表明,不同产地板蓝根药材其主要成分含量存在差异。结论:建立了板蓝根超微粉体双重指纹图谱,色谱图中各个峰分离度较好、特征明显,为现阶段板蓝根超微粉体质量控制提供了科学依据。
Objective: To establish the double HPLC fingerprints of water-soluble composition and amino acids precolumn derivative reagent of 13 batches of Isatidis Radix micropower.Method: The gradient elution was adopted with Hypersil BDS C18 column(4.6 mm×250 mm,5 μm).Water-soluble ingredients were detected with acetonitrile-0.1% phosphoric acid solution as mobile phase,flow rate 0.5 mL·min-1,column temperature 20 ℃,and the injection volume 10 μL.Amino acid ingredient were derived by PITC,and then were detected with mobile phase of 0.1 mol·L-1 sodium acetate buffer solution(pH 6.5)-acetonitrile,flow rate 1.0 mL·min-1,column temperature 30 ℃,and the injection volume 5 μL.Both of the absorption wavelengths were 254 nm.Pharmacopoeia Commission "Chinese chromatographic fingerprint evaluation system(version 2.0)" was adopted to analyse,fingerprints of Isatidis Radix micropower were established,at the same time 4 main ingredients were recognized by the SPSS cluster analysis.Result: Common mode of Fingerprint to water-soluble and amino acids ingredient of Isatidis Radix micropower was established,then adenosine,epigoitrin and 15 amino acids were identified as characteristic peaks.Cluster analysis showed that different kinds of the herbal Isatidis Radix micropower from different areas were different levels in the main ingredients.Conclusion: Double fingerprints of Isatidis Radix micropower is established.Each peak is optimally separated in chromatogram,which provides a scientific basis for quality control of Isatidis Radix micropower.

关键词(KeyWords): 板蓝根;超微粉体;水溶性成分;异硫氰酸苯酯;柱前衍生化;氨基酸;指纹图谱
Isatidis Radix;micropower;water-soluble ingredient;PITC;precolumn derivative reagent;amino acids;fingerprint

Abstract:

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基金项目(Foundation): 中医药行业科研专项(200807054)

作者(Author): 冯小燕;张水寒;蔡光先;黄一科;邵怡;
FENG Xiaoyan1,3,ZHANG Shuihan2,3,CAI Guangxian2,3,HUANG Yike1,3,SHAO Yi1,3(1.Hunan University of Chinese Medicine,Changsha 410013,China; 2.Hunan Academy of Chinese Medicine,Changsha 410013,China; 3.Hunan Innovation Team of Teachs hall Chinese Medicine Powder Technology,Hunan Key Laboratory of Chinese Medicine Powder and Innovation Drug Province Department Co-construction,Changsha 410013,China)

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