中国中药杂志

2015, v.40(16) 3239-3244

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“建莲”莲子心中生物碱的含量测定及HPLC指纹图谱的研究
Study on content determination of alkaloids and HPLC fingerprint of“Jianlian”Nelumbinis Plumula

刘久石;何燕峰;高石曼;张本刚;刘海涛;肖培根;
LIU Jiu-shi;HE Yan-feng;GAO Shi-man;ZHANG Ben-gang;LIU Hai-tao;XIAO Pei-gen;Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences,Peking Union Medical College;Tianjin Tasly TCM Technology Company Limited;

摘要(Abstract):

对"建莲"莲子心中3种生物碱类成分进行含量测定,并建立HPLC指纹图谱分析方法,用于"建莲"莲子心的化学质量评价。采用Thermo C18(4.6 mm×250 mm,5μm)为色谱柱,乙腈-十二烷基磺酸钠溶液-冰醋酸(56∶43∶1)为流动相,流速1.0 m L·min-1,检测波长282 nm,柱温35℃,对"建莲"莲子心中3种生物碱类成分进行含量测定。采用Agilent ZORBAX SBAq C18(4.6 mm×250 mm,5μm)为色谱柱,甲醇-水为流动相,流速0.8 m L·min-1,检测波长282 nm,柱温35℃,建立"建莲"莲子心的HPLC指纹图谱,并应用相似度评价和聚类分析的方法对12批"建莲"莲子心样品的HPLC指纹图谱数据进行分析。结果显示11批"建莲"莲子心样品在化学成分上具有较好的一致性。该方法较好地反映了"建莲"莲子心中的化学成分信息,且操作简单,重复性好,为进行"建莲"莲子心的化学质量评价提供了参考。
This study is to determine the content of three alkaloids and establish the HPLC fingerprint of "Jianlian"Nelumbinis Plumula. The HPLC method of content determination was as follows: Thermo C18( 4. 6 mm × 250 mm,5 μm) was conducted with acetonitrile-sodium dodecyl sulfonate solution-acetic acid( 56∶ 43∶ 1) at a flow rate of 1. 0 m L·min- 1. The monitoring wavelength was set at 282 nm and the column temperature was 35 ℃. The method of HPLC fingerprint was as follows: Agilent ZORBAX SB-Aq C18( 4. 6mm × 250 mm,5μm) was conducted with gradient elution of methanol and water at a flow rate of 0. 8 m L·min- 1,the monitoring wavelength was set at 282 nm and the column temperature was 35 ℃. Similarities evaluation and hierarchical clustering analysis were applied to demonstrate the variability of 12 batches of "Jianlian"Nelumbinis Plumula samples. The results demonstrated that 11 batches showed good similarity on chemical constituents. The method could well display the chemical information of"Jianlian"Nelumbinis Plumula. It was simple,reliable and could be used for the chemical quality control of "Jianlian"Nelumbinis Plumula.

关键词(KeyWords): “建莲”莲子心;含量测定;HPLC指纹图谱;聚类分析
"Jianlian"Nelumbinis Plumula;content determination;HPLC fingerprint;hierarchical cluster analysis

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作者(Author): 刘久石;何燕峰;高石曼;张本刚;刘海涛;肖培根;
LIU Jiu-shi;HE Yan-feng;GAO Shi-man;ZHANG Ben-gang;LIU Hai-tao;XIAO Pei-gen;Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences,Peking Union Medical College;Tianjin Tasly TCM Technology Company Limited;

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