中国中药杂志

2013, v.38(11) 1766-1771

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不同产地苍耳子UPLC指纹图谱研究
UPLC fingerprint of Xanthii Fructus from different habitats

洪燕;韩燕全;夏伦祝;桂洁;陈曦;孙艳华;
HONG Yan1,HAN Yan-quan2,XIA Lun-zhu2,GUI Jie1,CHEN Xi1,SUN Yan-hua1(1.School of Integrated Traditional and Western Medicine,Anhui University of Chinese Medicine,Hefei 230031,China; 2.The First Affiliated Hospital,Anhui University of Chinese Medicine,Grade 3 Laboratory of Traditional Chinese Medicine Preparation,State Administration of Traditional Chinese Medicine,Hefei 230031,China)

摘要(Abstract):

建立不同产地苍耳子药材UPLC指纹图谱,为其质量控制提供比较全面的评价方法。实验采用UPLC-PDA检测法,测定了26个产地的苍耳子药材进行UPLC指纹图谱,采用Acquity BEH C18色谱柱(2.1 mm×100 mm,1.7μm),乙腈-0.1%磷酸溶液为流动相梯度洗脱,流速为0.25 mL.min-1;检测波长220 nm。建立了26个产地苍耳子药材的共有图谱,确定了19个共有峰,对9个峰进行了指认;其中21批样品相似度大于0.9;26批药材可大致聚成6类;计算了各个指纹峰的主成分分值,6个主成分累计变量贡献值达到81.140%。该方法可用于苍耳子药材质量评价。
This study was establish an UPLC fingerprint of Xanthii Fructus from different habitats,to provide a comprehensive evaluation for its quality control.UPLC-PDA was adopted to analysis of 26 baches of Xanthii Fructus from different habitats.The chromatographic condition was as follow: ACQUITY BEH C18 Column(2.1 mm×100 mm,1.7 μm) eluted with the mobile phases of acetonitrile and 0.1% phosphoric acid water in gradient mode.The flow rate was 0.25 mL·min-1 and the detection wavelength was set at 220 nm.The fingerprints of 26 batches Xanthii Fructus were carried out by similarity comparation,cluster and the principal component analysis(PCA).There were nineteen common peaks,nine of which had been identified,and the similarity degrees of the twenty-six batches of the samples were between 0.804 and 0.990.All the samples were classified into six categories,and the PCA value of each fingerprint peak was calculated,and six principal components accounted for over 81.140% of the total variance were extracted from the original data.This method can be used to assess the quality of Xanthii Fructus.

关键词(KeyWords): 苍耳子;产地;UPLC;指纹图谱
Xanthii Fructus;different habitats;UPLC;chromatographic fingerprint

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基金项目(Foundation): 国家中医药重点学科临床中药学建设项目(国中医药人教发[2012]32号)

作者(Author): 洪燕;韩燕全;夏伦祝;桂洁;陈曦;孙艳华;
HONG Yan1,HAN Yan-quan2,XIA Lun-zhu2,GUI Jie1,CHEN Xi1,SUN Yan-hua1(1.School of Integrated Traditional and Western Medicine,Anhui University of Chinese Medicine,Hefei 230031,China; 2.The First Affiliated Hospital,Anhui University of Chinese Medicine,Grade 3 Laboratory of Traditional Chinese Medicine Preparation,State Administration of Traditional Chinese Medicine,Hefei 230031,China)

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