中国中药杂志

2019, v.44(08) 1615-1622

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女贞子中非三萜类成分的定性定量研究
Qualitative and quantitative analysis on non-triterpenoids in Ligustri Lucidi Fructus

纪鑫;刘晓谦;肖苏萍;杨立新;冯伟红;李春;王智民;
JI Xin;LIU Xiao-qian;XIAO Su-ping;YANG Li-xin;FENG Wei-hong;LI Chun;WANG Zhi-min;National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicines,Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences;China National of Traditional & Herbal Medicine Co.,Ltd.;

摘要(Abstract):

为了提高女贞子质量控制水平并探讨女贞子炮制后化学成分的变化,该文建立了女贞子中大极性成分的HPLC指纹图谱和多成分同步测定方法。以十八烷基硅烷键合硅胶为固定相,乙腈-0. 2%甲酸为流动相,梯度洗脱,检测波长280 nm,流速1. 0 m L·min-1,柱温25℃。女贞子药材指纹图谱中共选择出22个共有峰,酒女贞子中选出20个,采用对照品指认了二者共有的14个色谱峰。采用同一色谱条件对其中7个成分进行了同步测定,系统适应性与方法学考察结果均符合含量测定要求。与同批次女贞子相比,酒女贞子中5-羟甲基糠醛和红景天苷的含量大幅升高,而环烯醚萜苷类成分的含量普遍降低。建立的方法为女贞子药材、饮片和相关制剂的质量控制提供了依据。
In order to improve the quality control level of Ligustri Lucidi Fructus(LLF) and to explore the changes of chemical components after processing,the HPLC method for fingerprint and simultaneous determination of the major polar components in LLF were established. The octadecylsilane bonded silica gel was used as the stationary phase,with acetonitrile as the mobile phase A and0. 2% formic acid as the mobile phase B in a gradient elution procedure at a flow rate of 1. 0 m L·min-1. The detection wavelength was set at 280 nm and the column temperature was 25 ℃. There were 22 common peaks,20 of which were selected from the fingerprint of LLF and its wine-steamed product,respectively,and 14 chromatographic peaks were identified with reference substances. With the same chromatographic conditions,seven components were quantitatively analyzed and the results of system adaptability and methodology investigation all met the requirements of content determination. Compared with the crude LLF,the content of 5-hydroxymethyl furfural and salidroside significantly increased in wine-steamed LLF,while the contents of iridoid glycosides generally decreased. The method provided a basis for quality control of LLF and its processed products as well as the related preparations.

关键词(KeyWords): 指纹图谱;含量测定;高效液相色谱;环烯醚萜苷;苯乙醇苷;女贞子
fingerprint;content determination;high-performance liquid chromatography;iridoid glycosides;phenylethanoid glycosides;Ligustri Lucidi Fructus(LLF)

Abstract:

Keywords:

基金项目(Foundation): 国家中药标准化项目(ZYBZH-C-JL-26);; 北京市科技计划课题项目(Z171100001717028);; “中医药现代化研究”重点专项(SQ2017YFC170519)

作者(Author): 纪鑫;刘晓谦;肖苏萍;杨立新;冯伟红;李春;王智民;
JI Xin;LIU Xiao-qian;XIAO Su-ping;YANG Li-xin;FENG Wei-hong;LI Chun;WANG Zhi-min;National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicines,Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences;China National of Traditional & Herbal Medicine Co.,Ltd.;

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DOI: 10.19540/j.cnki.cjcmm.20190118.001

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