中国中药杂志

2018, v.43(14) 2964-2972

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基于内切-1,4-β-甘露聚糖酶水解的黄芪糖指纹图谱的建立及不同种质资源黄芪鉴别
Establishment of fingerprint of Astragali Radix polysaccharides based on endo-1,4-β-mannanase hydrolysis and identification of Astragali Radix of different germplasm resources

王迪;李科;王桂臻;李震宇;秦雪梅;杜冠华;张翔;
WANG Di;LI Ke;WANG Gui-zhen;LI Zhen-yu;QIN Xue-mei;DU Guan-hua;ZHANG Xiang;Modern Research Center for Traditional Chinese Medicine,Shanxi University;College of Chemistry and Chemical Engineering,Shanxi University;Key Laboratory of chemical Biology and Molecular Engineering Ministry,Shanxi University;Institute of Materia Medica,Chinese Academy of Medical Sciences & Peking Union Medical College;University of Louisville;

摘要(Abstract):

以内切-1,4-β-甘露聚糖酶的最佳酶解条件对不同种质资源蒙古黄芪(移栽芪和野生芪)和膜荚黄芪(移栽芪和野生芪)多糖进行酶解,通过荧光辅助糖电泳获得糖指纹图谱,利用主成分分析对数据进行分析,获得区分不同种质资源黄芪的差异性糖组分。结果显示,内切-1,4-β-甘露聚糖酶酶解产物中三糖、四糖和五糖可以作为区分蒙古黄芪(移栽芪和野生芪)的差异性糖片段,五糖和六糖可以作为区分膜荚黄芪(移栽芪和野生芪)的差异性糖片段,三糖和四糖可以作为区分移栽蒙古黄芪和移栽膜荚黄芪的差异性糖片段。研究表明,内切-1,4-β-甘露聚糖酶降解的多糖产物能很好地鉴别黄芪种属(蒙古黄芪与膜荚黄芪),生长方式(移栽芪和野生芪)。该研究为黄芪药材的品质评价及活性寡糖的筛选奠定了基础。
The polysaccharides of different germplasm resources of Astragalus membranaceus var. mongholicus(cultured Astragalus Radix(RA) and natural RA) and A. membranaceus(MJ)(cultured RA and natural RA) were studied by using the optimal enzymatic conditions of endo-1,4-β-mannanase. Saccharide fingerprints were obtained for the identification and evaluation of the germplasm resources of RA by Fluorophore-assisted Carbohydrate Electrophoresis(FACE). The data were analyzed by principal component analysis to obtain the difference between RA of different germplasm resources. The results showed that trisaccharide,tetrasaccharide and pentasaccharide of endo-1,4-β-mannanase hydrolyzate could be used as the differential fragments to distinguish MG(cultured RA and natural RA); the pentasaccharide and hexasaccharide can be used as differentially expressed carbohydrate fragments that distinguish MJ(cultured RA and natural RA); the trisaccharide and tetrasaccharide can be used as the differential fragments to distinguish the cultured MG and cultured MJ. Studies have shown that polysaccharide products degraded by endo-1,4-β-mannanase can well distinguish RA species(MG and MJ),growth mode(cultured RA and natural RA). This study laid the foundation for the quality evaluation of Astragalus medicinal herbs and screening of active oligosaccharides.

关键词(KeyWords): 内切-1,4-β-甘露聚糖酶;黄芪;多糖;指纹图谱;主成分分析;种质资源
endo-1,4-β-mannanase;Astragali Radix;polysaccharide;fingerprint;principal component analysis;germplasm resources

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(31200278);; 教育部博士点新教师类基金项目(20131401120006);; 山西省优秀人才科技创新项目(201705D211020);; 山西省中药产业重点科技攻关项目(201603D311101);; 国家中药标准化项目(ZYBZH-Y-JIN-34);; 山西省科技攻关项目(2014ZD0401)

作者(Author): 王迪;李科;王桂臻;李震宇;秦雪梅;杜冠华;张翔;
WANG Di;LI Ke;WANG Gui-zhen;LI Zhen-yu;QIN Xue-mei;DU Guan-hua;ZHANG Xiang;Modern Research Center for Traditional Chinese Medicine,Shanxi University;College of Chemistry and Chemical Engineering,Shanxi University;Key Laboratory of chemical Biology and Molecular Engineering Ministry,Shanxi University;Institute of Materia Medica,Chinese Academy of Medical Sciences & Peking Union Medical College;University of Louisville;

Email:

DOI: 10.19540/j.cnki.cjcmm.20180315.003

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