王伽伯;张学儒;肖小河;楚笑辉;周灿平;金城;鄢丹;
WANG Jiabo1,ZHANG Xueru1,2,XIAO Xiaohe1,CHU Xiaohui1,2,ZHOU Canping1,2,JIN Cheng1,YAN Dan1(1.China Military Institute of Chinese Materia Medica,302 Military Hospital,Beijing 100039,China;2.College of Pharmacy,Hunan University of Traditional Chinese Medicine,Changsha 410208,China)

• 1:解放军302医院解放军中药研究所
• 2:湖南中医药大学药学院

摘要(Abstract)：

对比研究市售及主产区34批大黄药材5种蒽醌单体含量及指纹图谱差异,从化学角度探讨传统大黄药材商品规格等级划分的科学合理性,为其行业标准的制定提供实验依据。结果表明,大黄药材的商品规格、等级与蒽醌类药效成分的含量相关性不强,与销售价格相关性也不明确;指纹图谱能够在一定程度上区分不同产地来源商品大黄药材,但不能有效区分同一产地不同商品规格等级的大黄药材。综合来看,传统道地产区大黄药材的蒽醌含量相对较高,提示传统按照产地划分大黄药材规格有一定的合理性,并从活性成分含量的角度佐证了传统对大黄道地药材产地的记载具有一定的科学性。本文首次发现,可根据大黄药材酸水解后大黄酸或大黄酚的多少,将药材区分为大黄酸型和大黄酚型2种类型,其中大黄酸型药材大多产于青海、西藏、川西、甘肃等传统道地产区,且文献报道大黄酸在多种药理作用方面以及药代动力学性质都优于大黄酚,初步认为大黄酸型药材可能是优质大黄药材。该结果为从化学信息的角度修订大黄药材的商品规格标准提供了参考。
The differences of 34 rhubarb samples collected on the market and at producing area were investigated by chemical analysis on the contents of anthraquinones and chromatographic fingerprints,in order to assess the rationality of the commercial specification of rhubarb.The results indicated that the commercial specification of rhubarb was not correlated to the contents of anthraquinones as well as the price.The chromatographic fingerprints of rhubarb samples from different producing area were dissimilar,while the commercial specifications were difficult to be separated.Generally,the rhubarb samples produced in famous-region contained more anthraquinones.This demonstrated rationality on the traditional records of the famous-region of rhubarb from a chemical view.In this study,it was firstly reported that rhubarb could be categorized into two types,chrysophanol-type and rhein-type,based on the proportion of the two constituents in the total anthraquinones after acid hydrohysis.It was found that the rhubarb samples of rhein-type were mostly produced in famous-regions,such as Qinghai,Xizang,West Sichuan and Gansu.The literatures reported that rhein was superior to chrysophanol at many pharmacological effects and pharmacokinetic properties.Hence,we primarily considered that rhein-type rhubarb might be high-quality.These results were helpful to improve the commercial specification of rhubarb from a view of chemical information.

关键词(KeyWords)： 大黄;药材;化学类型;商品规格;蒽醌;指纹图谱
rhubarb;medical material;chemotype;commercial specification;anthraquinones;chromatographic fingerprint

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(30973947);; 国家杰出青年科学基金项目(30625042);; 国家公益性行业科研专项基金项目(200807020)

作者(Author): 王伽伯;张学儒;肖小河;楚笑辉;周灿平;金城;鄢丹;
WANG Jiabo1,ZHANG Xueru1,2,XIAO Xiaohe1,CHU Xiaohui1,2,ZHOU Canping1,2,JIN Cheng1,YAN Dan1(1.China Military Institute of Chinese Materia Medica,302 Military Hospital,Beijing 100039,China;2.College of Pharmacy,Hunan University of Traditional Chinese Medicine,Changsha 410208,China)

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DOI:

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