中国中药杂志

2016, v.41(09) 1635-1639

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基于体外抗氧化活性的参芪通脉益智方提取工艺评价及其谱效关系的初步研究
Evaluation for extraction process of Shenqi Tongmai Yizhi particles based on antioxidant capacity in vitro and its spectrum-effect relation

张晓莉;刘宇灵;樊丽姣;王月亮;陈凯;李慧;
ZHANG Xiao-li;LIU Yu-ling;FAN Li-jiao;WANG Yue-liang;CHEN Kai;LI Hui;Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences;School of Chinese Materia Medica,Jilin Agricultural University;

摘要(Abstract):

以维生素C为阳性对照组,比较了参芪通脉益智方不同提取工艺的体外抗氧化活性,并采用灰关联度分析探讨提取物指纹图谱各特征峰对其体外抗氧化作用贡献大小。结果显示参芪通脉益智方水提法、水提醇沉法、醇提法、醇水双提法所得提取物的IC_(50)依次为0.801 4,0.859 1,0.796 1,0.918 0 g·L~(-1),其中醇提法提取物体外抗氧化活性最强,IC_(50)最小,是抗氧化活性成分的最优提取方法,当药材质量浓度达到一定程度时,自由基清除率与维生素C对照组相近。灰关联度分析得出各成分对抗氧化作用贡献大小顺序(按特征峰编号)4>3>33>53>9>10>11>34>15>59>8>61>52>20>42>18>29。谱效关系的初步探索,为中药复方提取工艺及药效物质基础提供了一定参考。
Based on DPPH method,the antioxidant activities of Shenqi Tongmai Yizhi particles with different extraction processes were compared. The contribution to the anti-oxidant capacity in vitro was explored by means of grey relational analysis on different chemical compositions in the fingerprint. The results showed that the IC_(50) concentration values of water extract,water extract from alcohol precipitation,alcohol extract,and alcohol and water extract were 0. 801 4,0. 859 1,0. 796 1,0. 918 0 g·L~(- 1); and the alcohol extract is the best method to extract antioxidative components,with the highest antioxidant activity and lowest IC_(50). When the mass concentration of the herbs reached a certain degree,its free radical clearance rate was similar to that of vitamin C control group. The order of different chemical contributions of constituents to the antioxidant activity in the fingerprint was 4 > 3 > 33 > 53 > 9 > 10 > 11 >34 > 15 > 59 > 8 > 61 > 52 > 20 > 42 > 18 > 29. The preliminary exploration for the spectrum efficiency relations provides reference for studying traditional Chinese medicine compound processing method and the pharmacodyamic material basis.

关键词(KeyWords): 抗氧化;DPPH;指纹图谱;灰关联度分析;谱效关系
antioxidant;DPPH;fingerprint;grey relational analysis;spectrum efficiency relations

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基金项目(Foundation): 2009年中医药行业科研专项(200907001-5);; 中国中医科学院自主选题项目(Z02063)

作者(Author): 张晓莉;刘宇灵;樊丽姣;王月亮;陈凯;李慧;
ZHANG Xiao-li;LIU Yu-ling;FAN Li-jiao;WANG Yue-liang;CHEN Kai;LI Hui;Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences;School of Chinese Materia Medica,Jilin Agricultural University;

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