中国中药杂志

2010, v.35(06) 669-672

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不同种质青果清热利咽化学组分的研究
Main chemical components for clearing heat and relieving sore throat in different germplasm of Canarium album

王恒;宋良科;汤昊;何海洋;代春初;李小锋;
WANG Heng,SONG Liangke*,TANG Hao,HE Haiyang,DAI Chunchu,LI Xiaofeng(Life Science and Engineering College of Southwest Jiaotong University,Chengdu 610031,China)

摘要(Abstract):

目的:不同种质青果指标性组分的筛选及其含量测定,为质量标准制定奠定基础。方法:通过查阅文献与定性实验筛选确定没食子酸、东莨菪内酯和滨蒿内酯为青果主要清热利咽化学组分。采用为2005年版药典五倍子项下色谱条件测定没食子酸含量。东莨菪内酯和滨蒿内酯采用Waters Symmetry-C18色谱柱(4.6 mm×250 mm,5μm);流动相乙腈-水-冰乙酸16∶84∶0.5;流速1.0 mL.min-1;柱温35℃;检测波长345 nm测定含量。结果:没食子酸、东莨菪内酯与滨蒿内酯分离度良好,标准曲线在检测范围内线性关系良好,回收率分别为100.22%,101.22%,100.42%,RSD均小于2.0%。没食子酸含量广东青果大果高于小果,四川则相反,总香豆素小果普遍高于大果,四川高于广东。结论:青果的清热利咽的功效可能由没食子酸、东莨菪内酯、滨蒿内酯的协同作用形成,药用时小果优于大果,为青果药材及其制剂的质量控制提供了可靠依据。
Objective:To screen the representing components in different germplasms of Canarium album and determine the content by HPLC for laying the foundation for the quality specification.Method: Gallic acid and scopoletin and scoparone were chosen as the main chemical components for clearing heat and relieving sore throat by reviewing literature and qualitative experiment.Gallic acid was determined according the method of Galla chinensis in Chinese pharmacopoeia.Scopoletin and scoparone were determined by HPLC on C18 column(4.6 mm×250 mm,5 μm) and the mobile phase was acetonitrile-water-acetic acid(16∶84∶0.5) with the detection wavelength at 345 nm and column temperature at 35 ℃,the flow rate was 1.0 mL·min-1.Result: The resolution is good for gallic acid and scopoletin and scoparone.The standard curve in detection range showed good linear relation,the average recoveries were 100.22%,101.22% and 100.42%,respectively,RSD were all less than 2.0%.The content of gallic acid in the large fruit C.album from Guangdong was higher than that in the small fruit C.album,while the result was contrary in samples from Sichuan.The content of total coumarins in the small fruit C.album was generally higher than that in the large fruit C.album,which from Sichuan was higher than those from Guangdong.Conclusion: The efficacies of clearing heat and relieving sore throat in C.album were formed by synergistic effect of gallic acid and scopoletin and scoparone.The small fruit C.album was better than the large fruit C.album as medicinal materials,the results provide reliable basis for quality control in medicinal materials and its preparation.

关键词(KeyWords): 青果;种质;清热利咽;东莨菪内酯;滨蒿内酯
Canarium album;germplasm;clearing heat and relieving sore throat;scopoletin;scoparone

Abstract:

Keywords:

基金项目(Foundation): 国家科技基础条件平台项目(2005DKA21000)

作者(Authors): 王恒;宋良科;汤昊;何海洋;代春初;李小锋;
WANG Heng,SONG Liangke*,TANG Hao,HE Haiyang,DAI Chunchu,LI Xiaofeng(Life Science and Engineering College of Southwest Jiaotong University,Chengdu 610031,China)

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