中国中药杂志

2011, v.36(22) 3075-3078

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道地产区不同产地厚朴种子的电泳指纹图谱研究
Study on electrophoretogram of Magnolia officinalis seeds from different locations

李晓琳;邵爱娟;陈敏;黄璐琦;
LI Xiaolin,SHAO Aijuan,CHEN Min,HUANG Luqi(Institute of Chinese Material Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China)

摘要(Abstract):

目的:研究道地产区湖北恩施5个不同产地厚朴种子的十二烷基磺酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)电泳图谱,以探索厚朴种子不同产地的鉴别方法,从而为厚朴的良种选育及种子质量评价提供依据。方法:应用SDS-PAGE方法比较不同产地厚朴种子的蛋白电泳图谱在谱带数目、谱带强度和相对分子质量等方面的差异。结果:5个产地的厚朴种子的电泳图谱存在明显产地差异。结论:该电泳方法可为厚朴种子的产地鉴别提供依据。
Objective: To find the identification method of seeds from different locations,we analyzed the SDS-PAGE electrophoretogram of Magnolia officinalis seeds from 5 different locations of Enshi district,Hubei province,the genuine producing area of M.officinalis,and compared band numbers,band intensity and protein molecular weight of different locations.The result showed that there were significant differences among seeds from different locations.9 bands with the molecular weight about 80.34,63.07,56.48,51.32,40.64,25.18,22.63,21.70,18.14 kDa were involved in all seeds electrophoretograms and could be as standard bands of M.officinalis seeds.Seeds from Badong county,Jianshi county and Enshi city had their own special bands which were very different from other locations.So this electrophresis method can be applied to identify different locations of M.officinalis seeds.

关键词(KeyWords): 厚朴;地理变异;种子蛋白;产地差异;十二烷基磺酸钠-聚丙烯酰胺凝胶电泳
Magnolia officinalis;geographic variation;seeds protein;location difference;SDS-PAGE

Abstract:

Keywords:

基金项目(Foundation): 国际科技合作项目(2009DFA31660)

作者(Author): 李晓琳;邵爱娟;陈敏;黄璐琦;
LI Xiaolin,SHAO Aijuan,CHEN Min,HUANG Luqi(Institute of Chinese Material Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China)

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