中国中药杂志

2015, v.40(07) 1347-1354

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UHPLC-MS/MS快速鉴别红花中的化学成分
Rapid identification of chemical composition in safflower with UHPLC-LTQ-Orbitrap

王松松;马艳;张毅;李德凤;杨洪军;梁日欣;
WANG Song-song;MA Yan;ZHANG Yi;LI De-feng;YANG Hong-jun;LIANG Ri-xin;School of Traditional Chinese Medicine,Capital Medical University;Institute of Chinese Materia Medica China,Academy of Chinese Medical,Sciences;

摘要(Abstract):

采用UHPLC-LTQ-Orbitrap高分辨质谱仪,对红花的成分进行快速分离,并根据MS和MS~n高分辨质谱信息,对其成分进行快速辨识,为中药材红花药效物质基础研究,及质量控制提供参考依据。线性轨道离子阱串联Orbitrap检测器在正负离子模式下进行化合物的一级全扫描及多级碎片扫描,获得化合物一级分子离子峰及二级碎片信息,通过对化合物分子式的推导(δ≤5)及碎片离子的解析,进行化合物定性分析。共检测到45个化合物,推导出分子式(6≤5),其中识别26个,经过标准物质验证的为5个化合物。
The UHPLC-LTQ-Orbitrap high resolution mass spectrometer was used to explore the chemical compositions in safflower.The rapid separation of the compositions was conducted by the UHPLC,following by high resolution full scan and MS2 scan,under the positive and negative ion mode.The chemical formula of compositions were deduced by full scan data in less than 5,then the potential structures were confirmed by the MS2 data.Forty-nine compounds were detected,of which 26 was identified,and 5 compounds was validated by the standard substances.

关键词(KeyWords): 红花;UHPLC-LTQ-Orbitrap;化学成分
safflower;UHPLC-LTQ-Orbitrap;chemical compositions

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基金项目(Foundation): 国家自然科学基金青年基金项目(81203005)

作者(Author): 王松松;马艳;张毅;李德凤;杨洪军;梁日欣;
WANG Song-song;MA Yan;ZHANG Yi;LI De-feng;YANG Hong-jun;LIANG Ri-xin;School of Traditional Chinese Medicine,Capital Medical University;Institute of Chinese Materia Medica China,Academy of Chinese Medical,Sciences;

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