中国中药杂志

2013, v.38(23) 4056-4060

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诱导培养条件显著改变甘草愈伤组织次生代谢产物的多样性
Significant impact of different induction conditions on metabolic diversity of callus cell lines of Glycyrrhiza sp.

刘凤彩;吕建明;吴秀祯;张卫;
LIU Feng-cai;LV Jian-ming;WU Xiu-zhen;ZHANG Wei;Department of Biochemical Engineering,School of Chemical Engineering,Tianjin University;Tianjin Acelbio Biotechnology Co. ,Ltd. ,Tianjin International Joint Academy of Biotechnology and Medicine;

摘要(Abstract):

采用化学分析和高效液相色谱(HPLC)相结合的方法评价典型愈伤组织诱导培养条件对甘草愈伤组织次生代谢产物多样性的影响,这些诱导培养条件包括甘草种属(胀果甘草和光果甘草)、外植体(胚轴和子叶)、光照条件(24 h光照和24 h黑暗)和2种激素组合。以化学成分总黄酮为指标,采用单因素方差分析法(One-way ANOVA)评价不同诱导培养条件下获得的甘草愈伤组织的总黄酮含量,结果显示甘草种属、外植体和光照条件对甘草愈伤组织中总黄酮的含量影响显著(P<0.05)。采用HPLC指纹图谱的差异度评价不同诱导培养条件对甘草愈伤组织次生代谢产物多样性的影响发现:诱导培养条件对愈伤组织的次生代谢产物的多样性有显著影响,激素组合(差异度=0.37)和光照条件(差异度=0.45)对甘草愈伤组织次生代谢产物的多样性影响最大。上述结果对选择合适的愈伤组织和细胞系培养生产甘草药用次生代谢产物或活性组分具有重要的指导意义。
The purpose of this study was to evaluate the impact of callus induction and culture conditions on secondary metabolic diversity of the callus cell lines of traditional Chinese medicinal plant Glycyrrhiza sp.( Glycyrrhiza) by combined chemical analysis and HPLC fingerprint. These callus induction conditions included two Glycyrrhiza species,two types of explants,light and dark conditions,and two combinations of hormones. The evaluation was firstly based on the contents of total flavonoids in the callus by chemical analysis and one way ANOVA. The content of total flavonoids in callus was significantly( P < 0. 05) influenced by Glycyrrhiza species,light condition,and the combination of hormones. The callus was further evaluated using diversity factor based on the comparison of HPLC fingerprints of these callus cell lines. Diversity factor varies significantly for calli induced under different conditions,with the highest being at 0. 45 under light condition and combination of hormones. These results provide important knowledge for the selection of suitable callus cell lines for the production of pharmacologically important secondary metabolites or bioactive fractions by in vitro culture of Glycyrrhiza sp.

关键词(KeyWords): 甘草;愈伤组织;药用次生代谢产物;HPLC;指纹图谱;差异度
Glycyrrhiza;callus;secondary metabolite;fingerprint;HPLC;diversity factor

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作者(Author): 刘凤彩;吕建明;吴秀祯;张卫;
LIU Feng-cai;LV Jian-ming;WU Xiu-zhen;ZHANG Wei;Department of Biochemical Engineering,School of Chemical Engineering,Tianjin University;Tianjin Acelbio Biotechnology Co. ,Ltd. ,Tianjin International Joint Academy of Biotechnology and Medicine;

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