中国中药杂志

2004, (09)

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安徽药菊耐盐突变体的筛选
Selection of sodium chloride tolerant mutants in Chrysanthemum morifolium in Anhui

薛建平,张爱民,高翔,盛玮
XUE Jian-ping, ZHANG Ai-ming, Gao Xiang, SHENG Wei(Department of Biology, Huaibei Coal Industry Teachers'College, Huaibei 23 5000, China)[

摘要(Abstract):

目的 :通过组织培养技术和EMS诱导突变相结合筛选出愈伤组织耐盐突变系。方法 :取药菊叶片诱导形成愈伤组织 ,用 0 .2 %和 0 .5 %EMS分别经浸渍法和滴加法处理 ,培养 15d后逐次转移到含NaCl 0 .5 % ,1.0 %和 1.5 %的筛选培养基上 ,进而获得耐盐愈伤组织突变体。结果与结论 :经耐盐性和耐盐稳定性检验 ,证明所获得的愈伤组织是突变体
Objective: To select the sodium chloride t ol erant mutants of the Chrysanthemum morifolium callus through tissue culture and EMS (ethylmethane sulfonate) treatment. Method: Ca lli were induced form the leaves of C.morifolium. The calli were treated wit h 0.2% and 0.5% EMS, respectively. After 15 days'culture, the calli were transp lanted to selection media with 0.5%,1.0% and 1.5% NaCl, and the sodium chloride tolerant mutants were selected out. Result and Conclusion: After tested the sodium chloride tolerant stability, the callus selected are found to be the mutants indeed. [

关键词(KeyWords): 药用菊花;甲基磺酸乙酯;诱变;耐盐突变体
Chrysanthemum morifolium; EMS; inductio n; NaCl tolerant mutants

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基金项目(Foundation): 安徽省“十五”重大科技专项 ( 0 180 3 0 0 4)

作者(Author): 薛建平,张爱民,高翔,盛玮
XUE Jian-ping, ZHANG Ai-ming, Gao Xiang, SHENG Wei(Department of Biology, Huaibei Coal Industry Teachers'College, Huaibei 23 5000, China)[

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