安徽药菊耐盐突变体的筛选Selection of sodium chloride tolerant mutants in Chrysanthemum morifolium in Anhui
薛建平,张爱民,高翔,盛玮
XUE Jian-ping, ZHANG Ai-ming, Gao Xiang, SHENG Wei(Department of Biology, Huaibei Coal Industry Teachers'College, Huaibei 23 5000, China)[
摘要(Abstract):
目的 :通过组织培养技术和EMS诱导突变相结合筛选出愈伤组织耐盐突变系。方法 :取药菊叶片诱导形成愈伤组织 ,用 0 .2 %和 0 .5 %EMS分别经浸渍法和滴加法处理 ,培养 15d后逐次转移到含NaCl 0 .5 % ,1.0 %和 1.5 %的筛选培养基上 ,进而获得耐盐愈伤组织突变体。结果与结论 :经耐盐性和耐盐稳定性检验 ,证明所获得的愈伤组织是突变体
Objective: To select the sodium chloride t ol erant mutants of the Chrysanthemum morifolium callus through tissue culture and EMS (ethylmethane sulfonate) treatment. Method: Ca lli were induced form the leaves of C.morifolium. The calli were treated wit h 0.2% and 0.5% EMS, respectively. After 15 days'culture, the calli were transp lanted to selection media with 0.5%,1.0% and 1.5% NaCl, and the sodium chloride tolerant mutants were selected out. Result and Conclusion: After tested the sodium chloride tolerant stability, the callus selected are found to be the mutants indeed. [
关键词(KeyWords):
药用菊花;甲基磺酸乙酯;诱变;耐盐突变体
Chrysanthemum morifolium; EMS; inductio n; NaCl tolerant mutants
基金项目(Foundation): 安徽省“十五”重大科技专项 ( 0 180 3 0 0 4)
作者(Author):
薛建平,张爱民,高翔,盛玮
XUE Jian-ping, ZHANG Ai-ming, Gao Xiang, SHENG Wei(Department of Biology, Huaibei Coal Industry Teachers'College, Huaibei 23 5000, China)[
Email:
DOI:
参考文献(References):
- [1] 王 杰,胡惠露,胡易冰,等.安徽药菊高产无公害栽培技术研究.中国中药杂志,2001,26(5):299.
- [2] 赵可夫,李法常.中国盐生植物.北京:科学出版社,1999.1.
- [3] 张继华,徐跃进,李晓丽,等.番茄耐盐突变体的筛选.西北植物学报,2002,22(2):257.
- [4] NaborsMW ,GibbsSE ,BernsteinCS ,etal.NaCl tolerenttobaccoplantsfromculturecell.Zpflanzenplyciol,1980,9:13.
- [5] 周荣仁,余叔文.烟草耐盐愈伤组织变异系的选择及生理生化特征的研究.植物生理学报,1993,19(2):188.
- [6] 陈可咏,叶和春,陈建林,等.芦苇耐盐变异植株及细胞学鉴定.植物学报,1994,36(12):930.
- [7] 郭丽娟.诱发玉米粒小叶斑病突变体的研究.遗传学报,1987,14(5):355.
- [8] 沈银柱,刘植义,何聪芬,等.诱发小麦花药愈伤组织及其再生植株抗盐性变异的研究.遗传,1997,19(6):7.
- [9] 张小玲,林蒙松,王元辉,等.烷化剂EMS处理水稻愈伤组织诱导突变的方法初探.安徽农业科学,1999,27(6):528.
- [10] 薛建平,于 淼,张爱民.安徽药菊叶片愈伤组织诱导及植株再生技术的研究.中国中药杂志,2003,28(3):213.
- [11] 邹 琦.植物生理学实验指导.北京:中国农业出版社,2000.159.
- [12] 陈 薇,李明扬,李宣源.离体茎尖诱变筛选油菜耐草酸变异体.西南农业学报.2001,14(2):31.
- [13] 廖树华.植物生物技术和作物改良.北京:中国科学技术出版社,1990.144.
- [14] 中国科学院上海植物生理研究所.现代植物生理学实验指南.北京:科学出版社,1999.358.
- [15] 王昆山,陆 卫,孙 彤,等.枸杞耐盐变异体的筛选及植株再生.遗传,1995,17(6):7.
- 薛建平
- 张爱民
- 高翔
- 盛玮
XUE Jian-ping - ZHANG Ai-ming
- Gao Xiang
- SHENG Wei(Department of Biology
- Huaibei Coal Industry Teachers'College
- Huaibei 23 5000
- China)[
- 薛建平
- 张爱民
- 高翔
- 盛玮
XUE Jian-ping - ZHANG Ai-ming
- Gao Xiang
- SHENG Wei(Department of Biology
- Huaibei Coal Industry Teachers'College
- Huaibei 23 5000
- China)[