中国中药杂志

2003, (07)

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色谱指纹图谱分析技术用于鉴别中药川芎产地
Identification of the Habitat of Ligusticum chuangxiong with Chromatographic Fingerprinting

陈闽军,吴永江,范骁晖,程翼宇
CHEN Min-jun, WU Yong-jiang, FAN Xiao-hui, CHENG Yi-yu (Department of Chinese Medicine Science and Engineering, Zhejiang University, Hangzhou 310027, Zhejiang, China)

摘要(Abstract):

目的:研究用于鉴别川芎产地的色谱指纹图谱分析技术。方法:用HPLC获得川芎药材的色谱图,从中提取15个色谱峰,再用主成分分析、聚类分析、相似度计算以及Fisher因子分析等方法进行考察。结果:不同产地川芎的色谱指纹图谱存在明显的差异,所提取的15个色谱峰可用作指纹特征。结论:色谱指纹图谱分析技术可用于川芎药材的产地鉴别。
Objective: To establish the chromatographic fingerprinting for identifying the habitat of Ligusticum chuangx-iong. Method: HPLC system was applied to obtain the chromatograms of L. chuangxiong samples from different areas, and 15 peaks were measured from the chromatograms. Then some computer-based methods including principle component analysis, clustering analysis, similarity calculation and fisher factor analysis were applied for data analysis. Result: There was obvious difference among chromatographic fingerprints of L. chuangxiong samples from different areas. The 15 measured peaks could be used as the fingerprint features. Conclusion: Chromatographic fingerprinting can be used for identifying the habitat of L. chuangxiong.

关键词(KeyWords): 中药;川芎;色谱指纹图谱;中药质量控制
traditional Chinese medicine (TCM); Ligusticum chuangxiong; chromatographic fingerprint; quality control of TCM

Abstract:

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基金项目(Foundation): 国家自然科学基金(39870940);; 国家重点基础研究发展规划(G1999054405);; 国家“十五”重大科技攻关计划(2001BA701A01)资助项目

作者(Author): 陈闽军,吴永江,范骁晖,程翼宇
CHEN Min-jun, WU Yong-jiang, FAN Xiao-hui, CHENG Yi-yu (Department of Chinese Medicine Science and Engineering, Zhejiang University, Hangzhou 310027, Zhejiang, China)

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