色谱指纹图谱分析技术用于鉴别中药川芎产地Identification of the Habitat of Ligusticum chuangxiong with Chromatographic Fingerprinting
陈闽军,吴永江,范骁晖,程翼宇
CHEN Min-jun, WU Yong-jiang, FAN Xiao-hui, CHENG Yi-yu (Department of Chinese Medicine Science and Engineering, Zhejiang University, Hangzhou 310027, Zhejiang, China)
摘要(Abstract):
目的:研究用于鉴别川芎产地的色谱指纹图谱分析技术。方法:用HPLC获得川芎药材的色谱图,从中提取15个色谱峰,再用主成分分析、聚类分析、相似度计算以及Fisher因子分析等方法进行考察。结果:不同产地川芎的色谱指纹图谱存在明显的差异,所提取的15个色谱峰可用作指纹特征。结论:色谱指纹图谱分析技术可用于川芎药材的产地鉴别。
Objective: To establish the chromatographic fingerprinting for identifying the habitat of Ligusticum chuangx-iong. Method: HPLC system was applied to obtain the chromatograms of L. chuangxiong samples from different areas, and 15 peaks were measured from the chromatograms. Then some computer-based methods including principle component analysis, clustering analysis, similarity calculation and fisher factor analysis were applied for data analysis. Result: There was obvious difference among chromatographic fingerprints of L. chuangxiong samples from different areas. The 15 measured peaks could be used as the fingerprint features. Conclusion: Chromatographic fingerprinting can be used for identifying the habitat of L. chuangxiong.
关键词(KeyWords):
中药;川芎;色谱指纹图谱;中药质量控制
traditional Chinese medicine (TCM); Ligusticum chuangxiong; chromatographic fingerprint; quality control of TCM
基金项目(Foundation): 国家自然科学基金(39870940);; 国家重点基础研究发展规划(G1999054405);; 国家“十五”重大科技攻关计划(2001BA701A01)资助项目
作者(Author):
陈闽军,吴永江,范骁晖,程翼宇
CHEN Min-jun, WU Yong-jiang, FAN Xiao-hui, CHENG Yi-yu (Department of Chinese Medicine Science and Engineering, Zhejiang University, Hangzhou 310027, Zhejiang, China)
Email:
DOI:
- 中药
- 川芎
- 色谱指纹图谱
- 中药质量控制
traditional Chinese medicine (TCM) - Ligusticum chuangxiong
- chromatographic fingerprint
- quality control of TCM
- 陈闽军
- 吴永江
- 范骁晖
- 程翼宇
CHEN Min-jun - WU Yong-jiang
- FAN Xiao-hui
- CHENG Yi-yu (Department of Chinese Medicine Science and Engineering
- Zhejiang University
- Hangzhou 310027
- Zhejiang
- China)
- 陈闽军
- 吴永江
- 范骁晖
- 程翼宇
CHEN Min-jun - WU Yong-jiang
- FAN Xiao-hui
- CHENG Yi-yu (Department of Chinese Medicine Science and Engineering
- Zhejiang University
- Hangzhou 310027
- Zhejiang
- China)