中国中药杂志

2012, v.37(11) 1610-1613

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不同产地通关藤药材HPLC-ELSD指纹图谱
HPLC-ELSD fingerprint of Marsdenia tenacissima from different habitats

李迩娜;滕再进;刘世平;钦松;张仓;
LI Erna,TENG Zaijin,LIU Shiping,QIN Song,ZHANG Cang*(Nanjing Sanhome pharmaceutical Co.,Ltd.,Nanjing 210038,China)

摘要(Abstract):

目的:建立不同产地通关藤药材HPLC-ELSD指纹图谱,为科学评价和有效控制其质量提供可靠的方法。方法:采用HPLC-ELSD测定了25个来自不同产地的通关藤药材样品,采用药典委员会颁布的中药色谱指纹图谱相似度软件评价系统2004A进行评价,建立了共有模式图,以相关度评价图谱的相似性。结果:建立了通关藤药材C21甾体类指纹图谱的共有模式,包含11个共有特征峰,对11个共有峰中10个峰进行了指认。以共有模式对25批药材进行相似度评价,其中80%的样品甾体HPLC-ELSD指纹图谱的相似度大于0.80。结论:本方法可用于通关藤药材质量评价。
Objective: To establish a HPLC-ELSD fingerprint of Marsdenia tenacissima from different habitats,in order to provide a reliable method for scientific assessment and effective quality control.Method: HPLC-ELSD was adopted to determine 25 baches of M.tenacissima herbs from different habitats.Traditional Chinese medicine chromatographic fingerprint similarity software assessment system 2004 developed by China Pharmacopoeia Committee was adopted to establish a common mode chart and assess chromatographic similarity based on the degree of correlation.Result: The common mode for M.tenacissima herb C21 steroidal fingerprint was established,including 11 common characteristic peaks.Among them,10 were identified.According to the assessment on the similarity of 25 batches of samples,80% of them showed a similarity of over 0.80 in steroidal HPLC-ELSD fingerprint.Conclusion: The method can be used to assess the quality of M.tenacissima.

关键词(KeyWords): 通关藤;产地;HPLC-ELSD;指纹图谱
Marsdenia tenacissima;habitat;HPLC-ELSD;chromatographic fingerprint

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基金项目(Foundation): 国家“重大新药创制”科技重大专项(2011ZX09201-201-02)

作者(Author): 李迩娜;滕再进;刘世平;钦松;张仓;
LI Erna,TENG Zaijin,LIU Shiping,QIN Song,ZHANG Cang*(Nanjing Sanhome pharmaceutical Co.,Ltd.,Nanjing 210038,China)

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