中国中药杂志

2008, (19) 2212-2214

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天然虫草与虫草菌丝体的HPLC指纹图谱研究
HPLC fingerprint analysis of Cordyceps and mycelium of cultured cordy

武彦舒;周丹蕾;鄢丹;任永申;方艺霖;肖小河;杜晓曦;王建;
WU Yan-shu1,2,ZHOU Dan-lei1,3,YAN Dan1,REN Yong-shen1,2,FANG Yi-lin1,2,XIAO Xiao-he1,DU Xiao-xi4,WANG Jian2(1.PLA Institute of Chinese Materia Medica,302 Hospital of PLA,Beijing 100039,China;2.Pharmacy College,Chengdu University of Traditional Chinese Medicine,Chengdu 610075,China;3.Life Science and Technology College,Kunming University of Science and Technology,Kunming 650224,China;4.Centre for Drug Evaluation,SFDA,Beijing 100038,China)

摘要(Abstract):

目的:建立虫草及虫草菌丝体的HPLC指纹图谱,为其质量控制提供参考依据。方法:采用HPLC法,以磷酸缓冲盐(pH 6.5,A)-甲醇(B)为流动相,梯度洗脱,检测波长260 nm;对指纹图谱进行相似度评价、聚类分析。结果:所建立的指纹图谱分析方法的方法学考察符合规定标准,确定了10个共有峰;该方法能很好的鉴别不同产地虫草及虫草菌丝体。结论:该方法简便可靠,重复性好,可作为目前虫草内在质量控制的有效方法之一。
Objective: To establish HPLC fingerprints of cordyceps and mycelium of cultured cordy for quality control.Method: The analysis was carried out on a Diamonsil C18 column eluted with phosphate buffer(pH 6.5) and methanol as mobile phase,the gradient elution program was used,and the detection wavelength was 260 nm;The similarity of fingerprints was evaluated,then,cluster analysis was studied.Result: The method of HPLC fingerprint analysis was validated and in keeping with the requirement.Ten common peaks were found in HPLC fingerprint.Cordyceps from different areas and mycelium of cultured cordy could be distinguished by the HPLC fingerprint.Conclusion: All results above exhibit that this method is practicable,reproducible,and reliable as a method for the quality control of cordyceps and mycelium of cultured cordy at present.

关键词(KeyWords): 虫草;虫草菌丝体;HPLC指纹图谱;相似度评价;聚类分析;质量控制
cordyceps;mycelium of cultured cordy;HPLC fingerprint;evaluation of the similarity;hierarchical cluster analysis;quality control

Abstract:

Keywords:

基金项目(Foundation): 国家杰出青年科学基金(C03050204)

作者(Author): 武彦舒;周丹蕾;鄢丹;任永申;方艺霖;肖小河;杜晓曦;王建;
WU Yan-shu1,2,ZHOU Dan-lei1,3,YAN Dan1,REN Yong-shen1,2,FANG Yi-lin1,2,XIAO Xiao-he1,DU Xiao-xi4,WANG Jian2(1.PLA Institute of Chinese Materia Medica,302 Hospital of PLA,Beijing 100039,China;2.Pharmacy College,Chengdu University of Traditional Chinese Medicine,Chengdu 610075,China;3.Life Science and Technology College,Kunming University of Science and Technology,Kunming 650224,China;4.Centre for Drug Evaluation,SFDA,Beijing 100038,China)

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