中国中药杂志

2018, v.43(06) 1182-1187

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毛冬青HPLC-UV指纹图谱与化学模式识别
HPLC-UV fingerprints and chemical pattern recognition of Ilicis Pubescentis Radix

朱明娟;邝国俊;高巍;易欢;赵钟祥;周园;韦品清;廖琼峰;张蕾;
ZHU Ming-juan;KUANG Guo-jun;GAO Wei;YI Huan;ZHAO Zhong-xiang;ZHOU Yuan;WEI Pin-qing;LIAO Qiong-feng;ZHANG Lei;College of Chinese Traditional Medicine,Guangzhou University of Chinese Medicine;Guangzhou Institute for Drug Control;

摘要(Abstract):

为了建立毛冬青的高效液相(HPLC-UV)指纹图谱的质量评价方法。该文采用Phenomenex Luna C18色谱柱(4.6 mm×250 mm,5μm),以乙腈-0.05%磷酸水为流动相,梯度洗脱,流速为0.8 m L·min~(-1),柱温30℃,进样量10μL。检测波长210 nm,根据相似度评价,结合化学计量学方法对毛冬青进行质量评价。结果建立了16批毛冬青HPLC-UV指纹图谱,标定共有峰29个,通过对照品指认了12个共有峰,其中14批样品指纹图谱的相似度均>0.92,通过聚类分析可将样品聚为3类,结合主成分分析、正交偏最小二乘法-判别分析发现其中7个成分是造成不同批次样品差异性的主要标记物。根据色谱峰的紫外吸收特征,可将毛冬青药材的指纹图谱分为3个区:A区域色谱峰主要为木脂素及酚酸类成分;B区域色谱峰主要为皂苷类成分;C区域色谱峰主要为其他类成分。该文所建立的液相指纹图谱特征性和专属性强,可作为毛冬青药材内在质量控制的有效方法。
The present study is to establish the fingerprints for the quality evaluation of Ilicis Pubescentis Radix by HPLC-UV. The chromatographic conditions were defined as Phenomenex Luna C18( 4. 6 mm × 250 mm,5 μm). Mobile phase was acetonitrile-0. 05%phosphoric acid in gradient elution,and the flow rate was 0. 8 m L·min~(-1). Column temperature was 30 ℃ and the injection volume was10 μL. The detection wavelength was 210 nm. According to the similarity evaluation,the chemometric method was used to assess the quality of Ilicis Pubescentis Radix. The fingerprints of 16 batches of Ilicis Pubescentis Radix were established. There were 29 common peaks in the fingerprints and 12 common peaks were identified by reference substances. Fingerprints similarity of samples were greater than 0. 92. The samples were classified into three groups by hierarchical cluster analysis combined with principal component analysis( PCA) and orthogonal partial least squares-discriminant analysis( OPLS-DA),and seven components were the main markers that cause differences in the different batches of samples. By comparing the on-line UV spectra of chromatographic peaks,the chromatographic fingerprint was divided into three regions: region A showed seventeen main peaks( mainly lignans and phenolic acids); region B showed eight main peaks,which were proved as saponins; region C showed four main peaks,which were proved as other components. The established HPLC-UV fingerprint is highly specific,and can be used to evaluate the quality consistency of different batches of Ilicis Pubescentis Radix.

关键词(KeyWords): 毛冬青;指纹图谱;聚类分析;主成分分析;正交偏最小二乘法-判别分析
Ilicis Pubescentis Radix;fingerprints;cluster analysis;principal component analysis;orthogonal partial least squares-discriminant analysis

Abstract:

Keywords:

基金项目(Foundation): 广东省科技计划项目(2016A020226031);; 广州市科技计划项目(201607010334);; 广东省高水平大学建设专项——广东省教育厅特色创新项目(自然科学);; 国家自然科学基金项目(81673565,81270054)

作者(Author): 朱明娟;邝国俊;高巍;易欢;赵钟祥;周园;韦品清;廖琼峰;张蕾;
ZHU Ming-juan;KUANG Guo-jun;GAO Wei;YI Huan;ZHAO Zhong-xiang;ZHOU Yuan;WEI Pin-qing;LIAO Qiong-feng;ZHANG Lei;College of Chinese Traditional Medicine,Guangzhou University of Chinese Medicine;Guangzhou Institute for Drug Control;

Email:

DOI: 10.19540/j.cnki.cjcmm.2018.0043

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