中国中药杂志

2019, v.44(23) 5134-5142

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基于口尝评价和电子舌评价的药物苦味叠加规律研究
Study on superposition law of drug bitterness based on tongue taste evaluation and electronic tongue evaluation

李学林;张耀;陈鹏举;施钧瀚;姚静;王青晓;桂新景;张璐;王柯涵;马静;王艳丽;刘瑞新;
LI Xue-lin;ZHANG Yao;CHEN Peng-ju;SHI Jun-han;YAO Jing;WANG Qing-xiao;GUI Xin-jing;ZHANG Lu;WANG Ke-han;MA Jing;WANG Yan-li;LIU Rui-xin;Henan University of Chinese Medicine;Department of Pharmacy,The First Affiliated Hospital of Henan University of Chinese Medicine;Level Three Laboratory of Chinese Traditional Medical Preparation of National Administration of Traditional Chinese Medicine;Collaborative Innovation Center for Respiratory Disease Diagnosis and Treatment & Chinese Medicine Developmen

摘要(Abstract):

中药汤剂含有复杂的苦味。该文以中药单体苦味物质简单混合为切入点研究苦味叠加的呈苦规律。以盐酸小檗碱(生物碱类)、栀子苷(萜类)、熊果苷(糖苷类)为母液物质,以槐定碱(生物碱类)、龙胆苦苷(萜类)、葛根素(糖苷类)为添加物质,将不同的添加物质按照浓度梯度分别与不同母液两两混合进行苦味叠加得到混合液,通过口尝评价(THTPM)和电子舌评价添加物溶液和混合液的苦度,以威布尔、对数曲线建立添加物溶液及混合液的苦度-浓度拟合模型。对比分析添加物溶液与混合液的苦度-浓度模型、苦度差值(ΔI_0/ΔI_e),探讨母液对混合液苦度的影响规律。结果显示,添加物溶液苦度模型和混合液叠加苦度模型都符合威布尔模型和对数模型(口尝评价:R~2≥0. 887 0,P<0. 01;电子舌评价:R2≥0. 753 2,P<0. 05),威布尔模型的拟合度普遍高于对数模型;口尝苦度差值(ΔI_0)呈单调递减趋势;口尝叠加苦度和电子舌叠加苦度拟合方程:R~2≥0. 874 2,P<0. 01。该文通过对不同种类的中药苦味物质相互叠加,进行口尝和电子舌联合评价,发现叠加后的苦度依然与添加物质浓度呈威布尔或对数关系;口尝评价显示苦味母液对混合液苦度的影响随添加物浓度的升高而降低;电子舌叠加苦度和口尝叠加苦度明显相关。但后期需通过筛选浓度梯度、增大样本量进行更深入的验证。
Traditional Chinese medicine( TCM) decoction contains complex bitterness. In this paper,the simple mixing of TCM monomer bitter substances is used as the entry point to study the law of bitterness superposition. With berberine hydrochloride( alkaloids),geniposide( terpenoids),and arbutin( glycosides) as mother liquor,sophoridine( alkaloids),gentiopicroside( terpenoids),and puerarin( glycosides) as additive substances,these different additive substances were mixed with different mother liquor according to concentration gradients to form different liquid mixtures. The bitterness of the additive solution and the mixtures was evaluated by traditional human taste panel method( THTPM) and electronic tongue; the bitterness-concentration fitting model of the additive solution and the liquid mixtures was established by Weibull and logarithmic curves. By comparing and analyzing the bitterness-concentration model and the bitterness difference( ΔI_0/ΔI_e) of the additive solution and the mixture,the influence of mother liquor on the bitterness of the mixture was investigated. The results showed that both the additive solution bitterness model and the liquid mixture bitterness model were consistent with the Weibull model and the logarithmic model( THTPM: R~2≥0. 887 0,P<0. 01; electronic tongue test:R~2≥0. 753 2,P<0. 05). The fitting degree of the Weibull model was generally higher than that of the logarithmic model; the bitterness difference( ΔI_0) was monotonously decreasing; the fitting equation of tongue bitterness and electronic tongue bitterness: R~2≥0. 874 2,P<0. 01. In this article,through the superposition of different kinds of TCM bitter substances,THTPM and electronic tongue test was combined. It was found that the bitterness after superposition was still in Weibull or logarithmic relationship with the concentration of additive substances; THTPM showed that the effect of bitter mother liquor on the bitterness of the mixture decreased with the increase of the concentration of the additive; the bitterness of the electronic tongue was obviously related to the bitterness of THTPM. However,further verification is needed later by optimizing the concentration gradient and expanding the sample size.

关键词(KeyWords): 叠加苦度;呈苦规律;电子舌;口尝评价
superimposed bitterness;Bitter law;electronic tongue;tongue taste

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金面上项目(81774452);国家自然科学基金青年基金项目(81001646);; 河南省中医管理局中医药科学研究专项(2017ZY2039),河南省中医管理局首届河南省中医药传承与创新人才工程(仲景工程)项目(2019ZYBJ07);; 河南省科技攻关计划项目(172102310135)

作者(Author): 李学林;张耀;陈鹏举;施钧瀚;姚静;王青晓;桂新景;张璐;王柯涵;马静;王艳丽;刘瑞新;
LI Xue-lin;ZHANG Yao;CHEN Peng-ju;SHI Jun-han;YAO Jing;WANG Qing-xiao;GUI Xin-jing;ZHANG Lu;WANG Ke-han;MA Jing;WANG Yan-li;LIU Rui-xin;Henan University of Chinese Medicine;Department of Pharmacy,The First Affiliated Hospital of Henan University of Chinese Medicine;Level Three Laboratory of Chinese Traditional Medical Preparation of National Administration of Traditional Chinese Medicine;Collaborative Innovation Center for Respiratory Disease Diagnosis and Treatment & Chinese Medicine Developmen

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