中国中药杂志

2010, v.35(06) 741-744

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毒热平注射液对巨噬细胞TIR信号通路的影响
Effect of Dureping injection on TIR signal pathway on Ana-1 cells

张艳丽;王宁萍;顾立刚;李澎涛;
ZHANG Yanli1,WANG Ningping1,GU Ligang2,LI Pengtao2*(1.Department of Etiology and Immunology of Ningxia Medical University,Yinchuan 750004,China;2.Chinese Medicine Antivirus Lab of Beijing University of Chinese Medicine,Educational Departmentof China,Beijing 100029,China)

摘要(Abstract):

目的:研究毒热平注射液对流感病毒感染的小鼠腹腔巨噬细胞株Ana-1细胞TIR(Toll/IL-1 receptors)信号传导通路的影响。方法:用100 TCID50流感病毒亚甲型鼠肺适应株A/FM/1/47(H1N1)感染小鼠腹腔巨噬细胞株Ana-1后换用10,1 mg.L-1含药维持液继续培养,分别于12,24 h弃细胞上清液并收集细胞,提取巨噬细胞RNA,进行实时定量PCR反应(RT-PCR),动态测定毒热平注射液对病毒感染前后巨噬细胞TIR信号传导通路中各信号蛋白:Toll样受体7(TLR7)、髓样分化因子88(MyD88),IL-1受体相关激酶4(IRAK4)、肿瘤坏死因子相关激酶6(TRAF6)和核因子κB(NF-κB)mRNA表达水平的影响。结果:毒热平注射液可剂量依赖性地下调病毒感染巨噬细胞TLR7,MyD88,IRAK4,TRAF6,NF-κB mRNA的表达水平。结论:毒热平注射液能通过调节TIR信号传导通路各信号蛋白的活性发挥抗病毒感染作用。
Objective: To investigate the influence of Dureping injection to the murinal celiac macrophage Ana-1 on TIR signal pathway.Method: Ana-1 cell line was infected by influenza virus FM1 strain and treated with the Dureping injection in different concentrations(10,1 mg·L-1 group) for 12 h and 24 h.Then we collected the cells,extracted mRNA and measured the expressions of TLR7,MyD88,IRAK4,TRAF6 and NF-κB p65 respectively by RT-PCR.Result: Dureping injection down-regulated the expression of TLR7,MyD88,IRAK4,TRAF6 and NF-κB p65 mRNA in Ana-1 cell line infected by influenza virus,in a dose-dependent manner significantly.Conclusion: Dureping injection has an obvious effect against influenza virus FM1 strain by regulating the TIR signal pathway.

关键词(KeyWords): Ana-1;毒热平注射液;流感病毒;TIR;TLR7
Ana-1;Dureping injection;influenza virus;TIR;TLR7

Abstract:

Keywords:

基金项目(Foundation): 宁夏医科大学特殊人才启动项目(XT200802)

作者(Authors): 张艳丽;王宁萍;顾立刚;李澎涛;
ZHANG Yanli1,WANG Ningping1,GU Ligang2,LI Pengtao2*(1.Department of Etiology and Immunology of Ningxia Medical University,Yinchuan 750004,China;2.Chinese Medicine Antivirus Lab of Beijing University of Chinese Medicine,Educational Departmentof China,Beijing 100029,China)

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