中国中药杂志

2009, v.34(08) 1015-1017

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金银花不同干燥技术HPLC指纹图谱研究
Comparison on HPLC fingerprint profiles of Lonicera japonica bud obtained by various dryness techniques

熊艳;高慧敏;王智民;朱晶晶;
XIONG Yan,GAO Huimin,WANG Zhiming,ZHU Jingjing(Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China)

摘要(Abstract):

目的:对不同干燥技术所得金银花进行HPLC指纹图谱的比较研究。方法:Kromasil C18色谱柱(4.6 mm×250mm,5μm),乙腈-0.4%的磷酸水溶液梯度洗脱,检测波长238 nm,柱温30℃,流速1 mL.min-1。结果:建立了不同干燥技术处理的金银花高效液相指纹图谱,确定了17个共有峰,但是成分的相对含量有明显差异。结论:利用HPLC指纹图谱分析法可较全面的反映不同干燥技术处理的金银花间的差异。
Objective: To investigate ingredient differences of Lonicera japonica bud processed by different dryness techniques.Method: The HPLC fingerprint was used to evaluate the quality of L.japonica by various dryness process.The analysis was performed on a Kromasil C18(4.6 mm×250 mm,5 μm).Acetonitril and water containing 0.4% H3PO4 were used as mobile phases with gradient elution.The flow rate was set at 1.0 mL·min-1,the column temperature was set at 30 ℃,and the detection wavelength was at 238 nm.Result: The HPLC-fingerprint profiles of L.japonica from six different dryness techniques were determined.A total of 17 common peaks were found,but their contents from various dryness techniques were significantly different.Conclusion: HPLC-fingerprint technique was one of potential Process Analytical Techniques(PAT) to explore the ingredient differences in dryness process.

关键词(KeyWords): 金银花;干燥技术;绿原酸;木犀草苷;HPLC指纹图谱
Lonicera japonica;dryness technique;chlorogenic acid;luteoloside;HPLC-fingerprint profiles

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基金项目(Foundation): 国家“十一五”科技支撑计划项目(2006BAI09B05-9);; 国家自然科学基金项目(30672601)

作者(Author): 熊艳;高慧敏;王智民;朱晶晶;
XIONG Yan,GAO Huimin,WANG Zhiming,ZHU Jingjing(Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China)

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