苦参汤黄酮类成分HPLC指纹图谱及其与组方药味黄芩和苦参的相关性研究HPLC fingerprint of flavonoids of Kushen Tang and its correlation to Scutellaria baicalensis and Sophora flavescens
刘斌;石任兵;朱丽君;
LIU Bin,SHI Ren-bing,ZHU Li-jun(School of Chinese Pharmacology,Beijing University of Chinese Medicine,Beijing 100102,China)
摘要(Abstract)：
目的:建立苦参汤黄酮类成分的HPLC指纹图谱,研究该指纹图谱与组方药味黄芩和苦参的相关性。方法:采用HPLC方法,在检测波长280 nm和365 nm下,建立苦参汤黄酮类成分的指纹图谱;通过分析苦参汤不同配伍的HPLC图谱,探讨全方指纹图谱共有指纹峰与处方药味的相关性;采用添加对照品分析方法对共有指纹峰进行化学成分确认。结果:在检测波长280 nm下,苦参汤黄酮类成分的HPLC指纹图谱有19个共有指纹峰,除2个峰来源于苦参外,其余峰均来源于黄芩,其中8个峰分别为黄芩苷/三叶豆紫檀苷、汉黄芩苷、木犀草素、黄芩素、千层纸素A、5,8-二羟基-6,7-二甲氧基黄酮、汉黄芩素和芒柄花素/白杨素。在检测波长365 nm下,有22个共有指纹峰,13个峰来源于黄芩,6个峰来源于苦参,其中11个峰分别为黄芩苷、汉黄芩苷、黄芩素、千层纸素A、5,8-二羟基-6,7-二甲氧基黄酮、汉黄芩素、白杨素/7-甲氧基黄酮、2,′4-二羟基-4,′6′-二甲氧基查耳酮、4,4′-二甲氧基查耳酮、黄腐醇和苦参啶。结论:在检测波长280 nm和365 nm下获得的苦参汤黄酮类成分的HPLC指纹图谱共有指纹峰来源于组成药味黄芩和苦参,其中以365 nm为检测波长,建立苦参汤黄酮类成分的HPLC指纹图谱,更具代表性和全面性。
Objective:To establish the HPLC fingerprint of the flavonoids of Kushen Tang,and to study its correlation to Scutellaria baicalensis and Sophora flavescens.Method:The fingerprints of the flavonoids of Kushen Tang were established by HPLC under two detective wavelengths 280 nm and 365 nm.The correlation existed in the common peaks of HPLC fingerprint of Kushen Tang and the Chinese meteria medicines which composed the prescription was explored by analyzing the HPLC fingerprints of different compatibilities.The chemical constituents which the common peaks stood for were analyzed by adding controls to the samples respectively.Result:Under the detective wavelength 280 nm,19 common peaks were found,which came from S.baicalensis except that two peaks came from S.flavescens,and eight peaks were baicalin/trifolirhizin,wogonoside,luteolin,baicalein,oroxylin A,5,8-dihydroxy-6,7-dimethoxyflavone,wogonin and formononetin/chrysin respectively.Under the detective wavelength 365 nm,22 common peaks were found,which thirteen peaks came from S.baicalensis and six peaks came from S.flavescens.Among them,eleven peaks were baicalin,wogonoside,baicalein,oroxylin A,5,8-dihydroxy-6,7-dimethoxyflavone,wogonin,chrysin/7-methoxyflavone,2′,4-dihydroxy-4′,6′-dimethoxychalcone,4,4′-dimethoxychalcone,xanthohumol and kuraridin respectively.Conclusion:The common peaks of HPLC fingerprint of the flavonoids of Kushen Tang,which were obtained under the two detective wavelengths 280 nm and 365 nm,came from S.baicalensis and S.flavescens.It was more scientific and comprehensive that establishing the HPLC fingerprint of the flavonoids of Kushen Tang under the detective wavelength 365 nm.
关键词(KeyWords)：
苦参汤;黄酮;指纹图谱;黄芩;苦参
Kushen Tang;flavonoids;fingerprint;Scutellaria baicalensis;Sophora flavescens
基金项目(Foundation): 国家“十一五”科技支撑计划(2006BA108B03-04)
作者(Author):
刘斌;石任兵;朱丽君;
LIU Bin,SHI Ren-bing,ZHU Li-jun(School of Chinese Pharmacology,Beijing University of Chinese Medicine,Beijing 100102,China)
Email:
DOI:
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