中国中药杂志

2004, (11)

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HPLC测定金莲花制剂中荭草苷和牡荆苷的含量
Determination of orientin and vitexin in Trollius chinesis preparation by HPLC

刘智,王丽,李雯,黄勇,许祖超
LIU Zhi, WANG Li, LI Wen, HUANG Yong, XU Zu-chao (Guizhou-Eakan Pharmaceutical Co.,Ltd.,Guiyang 550001,China) [

摘要(Abstract):

目的 :建立金莲花制剂中荭草苷和牡荆苷的含量测定方法。方法 :采用高效液相色谱法 ,使用HypersilC18ODS色谱柱 ,流动相乙腈 1%醋酸溶液 (15∶85 ) ,流速 1 0mL·min-1,检测波长 340nm。结果 :荭草苷在 8 4 2~84 2 μg·mL-1呈良好的线性关系 (r=0 9999) ,平均回收率为 98 3% ,RSD =0 8% ;牡荆苷在 8 0 7~ 80 7μg·mL-1呈良好的线性关系 (r=0 9999) ,平均回收率为 10 2 4 % ,RSD =1 7%。结论 :本法灵敏、准确、专属性强 ,重现性好 ,可作为金莲花制剂的质量控制方法。
Objective: To establish a HPLC method for deter m ining orientin and vitexin in Trollius chinesis preparation. Method : The separation was crried out on Hypersil ODS column with a mixture o f acetonitrile-1% acetic acid solution (15∶85) as the mobile phase, at a flow rate of 1.0 mL·min -1 . The detection wavelength was set 340 nm. Result: Orientin showed a good linear relationship in the range of 8.42~84.2 μg·mL -1 (r=0.999 9) and the aver age recovery is 98.3%, RSD is 0.8%. Vitexin showed a good linear relationship in the range of 8.07 ~80.7 μg·mL -1 (r=0.999 9) and the average recovery is 102.4%. RSD is 1.7%. Conclusion: The method is sensitive, specific, and accurate with a good repeatability and can be used for the quality control of Trollius chinesis preparation. [

关键词(KeyWords): 金莲花制剂;荭草苷;牡荆苷;HPLC
Trollius chinesis; preparation; orientin; vitexin; HPLC

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作者(Author): 刘智,王丽,李雯,黄勇,许祖超
LIU Zhi, WANG Li, LI Wen, HUANG Yong, XU Zu-chao (Guizhou-Eakan Pharmaceutical Co.,Ltd.,Guiyang 550001,China) [

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