中国中药杂志

2006, (14) 1143-1146

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不同产地川芎中藁本内酯的含量及其质量指标
Determination of ligustilide for quality assessment of Ligusticum chuanxiong

程世琼;吕光华;梁士贤;王野;徐愚聪;赵中振;
CHENG Shi-qiong~(1),LV Guang-hua~(2,3),LIANG Shi-xian~2,WANG Ye~1,XU Yu-cong~1,ZHAO Zhong-zhen~2(1.Sichuan Provincial Institute for Food and Drug Control,Chengdu 610036,China;2.School of Chinese Medicine,Hong Kong Baptist University,Hong Kong,China;3.West China School of Pharmacy,Sichuan University,Chengdu 610041,China)

摘要(Abstract):

目的:测定不同产地川芎中藁本内酯的含量以控制其质量。方法:HPLC采用Alltima C18(4.6 mm×150 mm,5μm)色谱柱,以乙腈-水(60∶40)为流动相,流速为1.0 mL.min-1,柱温为室温,检测波长350 nm,以外标法测定川芎中藁本内酯的含量。结果:Z-藁本内酯在0.10~4.0μg呈线性关系,平均回收率为98.5%(n=5)。21份川芎原药材中Z-藁本内酯的平均质量分数为7.40 mg.g-1,即川芎原药材中Z-藁本内酯的含量以干燥品计,应不少于0.66%。结论:本方法简便,准确,重复性好,可用于川芎中藁本内酯的含量测定。不同产区川芎中藁本内酯的含量差异较大,道地产区药材中藁本内酯的含量较高。
Objective: To assay ligustilide content in the herb of Szechwan Lovage Rhizome(Chuanxiong,CX),which is the dried rhizome of Ligusticum chuanxiong in order to assess the quality.Method: Ligustilide was quantitatively analyzed by high-performance liquid chromatography in 21 CX samples.An Alltima C_(18) column(4.6 mm×150 mm,5 μm) was used as the analytical column.The mobile phase consisted of water and acetonitrile (40∶60).The flow rate was maintained at 1.0 mL·min~(-1) with the column temperature at ambient conditions.The detection wavelength was set at 350 nm.Result: The average content of Z-ligustilide in 21 CX samples was found to be 7.40±3.54 mg·g~(-1)(±s,n=21).Therefore,the content of Z-ligustilide in CX should not be less than 0.66%(calculated on the dried basis).Conclusion: The overall analytical procedure is rapid and accuracy which is considered suitable for the quantitative analysis of ligustilide in CX.The amount of ligustilide in CX samples collected from different cultivation areas was obviously different.However,a relatively higher content of ligustilide was generally found in the CX collected from its main cultivated areas.

关键词(KeyWords): 川芎;藁本内酯;高效液相色谱法
Ligusticum chuanxiong;ligustilide;HPLC

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作者(Author): 程世琼;吕光华;梁士贤;王野;徐愚聪;赵中振;
CHENG Shi-qiong~(1),LV Guang-hua~(2,3),LIANG Shi-xian~2,WANG Ye~1,XU Yu-cong~1,ZHAO Zhong-zhen~2(1.Sichuan Provincial Institute for Food and Drug Control,Chengdu 610036,China;2.School of Chinese Medicine,Hong Kong Baptist University,Hong Kong,China;3.West China School of Pharmacy,Sichuan University,Chengdu 610041,China)

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