中国中药杂志

2010, v.35(06) 750-754

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石斛对胃热证大鼠的作用及机制研究
Effects and mechanisms of Herba Dendrobii on rats with stomach-heat syndrome

李续娥;黄雪群;李小梅;
LI Xu′e1,HUANG Xuequn1,2,LI Xiaomei1,3*(1.Guangdong Province Key Lab of Biotechnology for Plant Development,South China NormalUniversity,Guangzhou 510631,China;2.Qingyuan Polytechnic College,Qingyuan 511510,China;3.Guangzhou University,Guangzhou 510511,China)

摘要(Abstract):

目的:探讨石斛对胃热证大鼠的作用及其机制。方法:用干姜水煎剂连续灌胃大鼠15 d,复制胃热证大鼠模型,再以石斛水煎剂(1.5,0.75 g.kg-1)灌胃治疗10 d。比较大鼠的饮食量、大小便、舌色舌苔变化,用红外热扫描成像(therm altexture m aps,TTM)系统检测大鼠全身不同部位的温度改变,用放射免疫分析法测定大鼠血液中与胃机能相关的生化指标血栓素B2(TXB2)、6-酮-前列腺素F1α(6-keto-PGF1α)、胃动素(MTL)、胃泌素(Gas)、生长抑素(SS)、白细胞介素-4(IL-4)、白细胞介素-8(IL-8),观察大鼠胃黏膜组织病理学变化情况。结果:造模后大鼠呈现舌红苔黄(P<0.05)、饮食量增加(10%以上)、大小便量减少(10%以上)、大便多数干结、小便颜色加深(P<0.01),大鼠的头、颈、左腋下、胸、上腹、中腹部体温显著升高(差值>0.5℃或差值>1.0℃);大鼠血液中显著降低的有6-keto-PGF1α(P<0.01),显著升高的有MTL,Gas,IL-8(P<0.01);大鼠胃黏膜受到损伤,呈现炎性变化(P<0.05,P<0.01)。石斛治疗后除食量,头、颈、胸部温度,血浆MTL浓度,胃黏膜腺体主细胞数外,上述胃热证的其他指标均得到改善(差值>0.5℃或差值>1.0℃,P<0.05,P<0.01)。结论:石斛通过使胃热证大鼠血液中的IL-8,Gas,TXB2降低,6-keto-PGF1α升高,改善胃热证大鼠的全身体征,发挥对大鼠胃黏膜的保护作用。
Objective: To observe the effects of Herba dendrobii on rats with stomach-heat syndrome and to explore the mechanisms.Method: Rats were fed with decoction of Rhizoma Zingiberis for 15 continuous days to induce the model of stomach-heat syndrome.After modeling,Herba Dendrobii(HD) decoction were given(in the doses of 1.5,0.75 g·kg-1 respectively) for 10 days.After treatment,amount of the daily diet,volume and absorbance of urine,pellet number and moistness of excrement,color and coating degree of tongue were recorded;the body thermal effects were detected with thermal texture maps(TTM) system;the biochemical indexes of blood reflecting the physiological function of stomach,including thromboxaneB2(TXB2),6-keto-prostaglandin F1α(6-keto-PGF1α),motilin(MTL),gastrin(Gas),somatostation(SS),interleukin-4(IL-4) and interleukin-8(IL-8) were measured by radioimmunoassay;and the histological changes of gastric mucosa were observed by hematoxylin-eosin(HE) stain.Result: The model rats had yellow coating and red tongues(P<0.05).The amount of daily diet were increased(over 10%),urine volume and excrement pellet number were decreased(over 10%).The their urine color became deep(P<0.01) and their excrement became dry.The temperatures in head,neck,left fore-armpit,chest,up-abdomen,mid-abdomen of the model rats were raised up(difference>0.5 ℃ or difference>1.0 ℃).The content of 6-keto-PGF1α in blood of model rats decreased evidently(P<0.01),and the contents of MTL,Gas and IL-8 increased conspicuously(P<0.01).The histological changes of gastric mucosa in the model rats were as follows: diffuse congestion,infiltration of neutrophil,less secretion,decrease of the number of chief and parietal cells,etc(P<0.05 or P<0.01).After treatment with HD,except the daily food weight,the temperatures in head,neck and chest,the content of MTL and the number of chief cells,the other indexes observed above were improved noticeably(difference>0.5 ℃ or difference>1.0 ℃,P<0.05 or P<0.01).Conclusion: The reason why HD relieves the general symptom and sign the gastric mucosa of rats with stomach-heat syndrome is that HD can increase 6-keto-PGF1α and decrease IL-8,Gas,TXB2 in their blood.

关键词(KeyWords): 石斛;胃热证;全身体征;胃部生理机能;作用机制
Herba Dendrobii;stomach-heat syndrome;general symptom and sign;gastric physiological function;effect mechanism

Abstract:

Keywords:

基金项目(Foundation): 广东省自然科学基金项目(7005844)

作者(Authors): 李续娥;黄雪群;李小梅;
LI Xu′e1,HUANG Xuequn1,2,LI Xiaomei1,3*(1.Guangdong Province Key Lab of Biotechnology for Plant Development,South China NormalUniversity,Guangzhou 510631,China;2.Qingyuan Polytechnic College,Qingyuan 511510,China;3.Guangzhou University,Guangzhou 510511,China)

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