马云桐;万德光;宋良科;
MA Yun-tong~1,WAN De-guang~1,SONG Liang-ke~2(1.Pharmacy School of Chengdu University of Traditional Chinese Medicine,Chengdu 610075,China;2.Pharmacy School of Southwest Jiaotong University,Emei 642002,China)

• 1:成都中医药大学药学院
• 2:成都中医药大学药学院
• 3:西南交通大学药学院 四川成都610015
• 4:四川成都610015

摘要(Abstract)：

目的:建立虎杖药材HPLC指纹图谱测定方法。方法:采用Diamonsil C18柱,流动相为乙腈-0.1%磷酸水溶液线性梯度洗脱,检测波长为230 nm。采用中南大学出版的指纹图谱相似度比较软件进行比较。结果:运用梯度洗脱能很好分离虎杖的各类成分,通过软件的比较,虎杖药材的指纹图谱相似度均大于0.80。结论:本研究所建立的方法可作为虎杖药材质量标准制定参考依据。
Objective: To establish the HPLC fingerprint of Rhizoma Polygoni Cuspidati(Polygonum cuspidatum).Method: The HPLC separation was carried with Diamonsil C_(18) column and eluted with a gradient from methanol and 0.1%phosphoric acid,the detection wavelength was at 230 nm and recording 70 min.The similarity of chromatograms was compared by mean of the software from Zhongnan University.Result: The constituents of P.cuspidatum were well separated by HPLC,and the similarity was above 0.80.Conclusion: The method can be used for the study of fingerprints of Rhizoma Polygoni Cuspidati.

关键词(KeyWords)： 虎杖;HPLC;指纹图谱
Polygonum cuspidatum;HPLC;fingerprint

Abstract:

Keywords:

基金项目(Foundation): 四川省科技厅重点科技项目(04NG020-03102)

作者(Author): 马云桐;万德光;宋良科;
MA Yun-tong~1,WAN De-guang~1,SONG Liang-ke~2(1.Pharmacy School of Chengdu University of Traditional Chinese Medicine,Chengdu 610075,China;2.Pharmacy School of Southwest Jiaotong University,Emei 642002,China)

Email:

DOI:

参考文献(References)：

• [1]肖培根.新编中药志.第1卷.北京:化学工业出版社,2002.579.
• [2]肖凯,宣利江,徐亚明,等.虎杖的化学成分研究.中国药学杂志,2003,38(1):12.
• [3]中国药典.一部.2005.145.