中国中药杂志

2006, (12) 972-974

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虎杖药材HPLC指纹图谱研究
HPLC fingerprint of Rhizoma Polygoni Cuspidati

马云桐;万德光;宋良科;
MA Yun-tong~1,WAN De-guang~1,SONG Liang-ke~2(1.Pharmacy School of Chengdu University of Traditional Chinese Medicine,Chengdu 610075,China;2.Pharmacy School of Southwest Jiaotong University,Emei 642002,China)

摘要(Abstract):

目的:建立虎杖药材HPLC指纹图谱测定方法。方法:采用Diamonsil C18柱,流动相为乙腈-0.1%磷酸水溶液线性梯度洗脱,检测波长为230 nm。采用中南大学出版的指纹图谱相似度比较软件进行比较。结果:运用梯度洗脱能很好分离虎杖的各类成分,通过软件的比较,虎杖药材的指纹图谱相似度均大于0.80。结论:本研究所建立的方法可作为虎杖药材质量标准制定参考依据。
Objective: To establish the HPLC fingerprint of Rhizoma Polygoni Cuspidati(Polygonum cuspidatum).Method: The HPLC separation was carried with Diamonsil C_(18) column and eluted with a gradient from methanol and 0.1%phosphoric acid,the detection wavelength was at 230 nm and recording 70 min.The similarity of chromatograms was compared by mean of the software from Zhongnan University.Result: The constituents of P.cuspidatum were well separated by HPLC,and the similarity was above 0.80.Conclusion: The method can be used for the study of fingerprints of Rhizoma Polygoni Cuspidati.

关键词(KeyWords): 虎杖;HPLC;指纹图谱
Polygonum cuspidatum;HPLC;fingerprint

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基金项目(Foundation): 四川省科技厅重点科技项目(04NG020-03102)

作者(Author): 马云桐;万德光;宋良科;
MA Yun-tong~1,WAN De-guang~1,SONG Liang-ke~2(1.Pharmacy School of Chengdu University of Traditional Chinese Medicine,Chengdu 610075,China;2.Pharmacy School of Southwest Jiaotong University,Emei 642002,China)

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