中国中药杂志

2009, v.34(06) 727-730

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络石藤高效液相色谱指纹图谱研究
Study on HPLC fingerprint of Trachelospermum jasminoides

刘义全;于能江;杨学东;赵毅民;
LIU Yiquan1,YU Nengjiang2,YANG Xuedong1,ZHAO Yimin2(1.School of Pharmaceutical and Technology,Tianjin University,Tianjin 300072,China;2.Institute of pharmacology and toxicology,Academy of Military Medical Sciences,Beijing 100850,China)

摘要(Abstract):

目的:建立络石藤药材的高效液相指纹图谱,以评价络石藤药材的质量。方法:利用HPLC-VWD方法,梯度洗脱,测定了14批络石藤样品。色谱条件为:ZORBAX Eclipse XDB-C18色谱柱(4.6 mm×150 mm,5μm),柱温30℃;流动相A相为水,B相为甲醇,梯度洗脱(10%~60%B),分析时间60 min;流速0.8 mL.min-1,检测波长230 nm。结果:14批络石藤样品得到的高效液相指纹图谱有19个共有峰,经相似度计算,14批药材整体相似性较好,其中10批药材之间的相似度较高,另外4批药材相似度偏低。结论:络石藤药材的指纹图谱特征性及专属性强,可用于全面控制络石藤药材的质量。
Objective:To establish HPLC fingerprint for identification and evaluation of the quality of Trachelospermum jasminoides.Method:The analysis was performed on a ZORBAX Eclipse XDB-C18 analytical column(4.6 mm×150 mm,5 μm) with H2O(A) and methanol(B) as mobile phases in gradient mode.The elutin conditions were 0-15 min,changed from 10% B to 30% B;15-40 min,to 40% B;40-60 min,to 60% B.The column temperature was set at 30 ℃ and the flow rate was 0.8 mL·min-1 with the detection wavelength of 230 nm.Result:The HPLC chromatographic fingerprint of T.jasminoides,showing 19 characteristic peaks,was established from 14 lots of T.jasminoides.The similarity of every lot of T.jasminoides was calculated as good in general,the similarity of 10 lots was above 0.9 and the other 4 lots had low similarity.Conclusion:The chromatographic fingerprint of T.jasminoides with high characteristics and specificity can be used to control its quality.

关键词(KeyWords): 络石藤;高效液相色谱;指纹图谱
Trachelospermum jasminoides;HPLC;fingerprint

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基金项目(Foundation): 国家自然科学基金面上项目(30300435)

作者(Author): 刘义全;于能江;杨学东;赵毅民;
LIU Yiquan1,YU Nengjiang2,YANG Xuedong1,ZHAO Yimin2(1.School of Pharmaceutical and Technology,Tianjin University,Tianjin 300072,China;2.Institute of pharmacology and toxicology,Academy of Military Medical Sciences,Beijing 100850,China)

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