中国中药杂志

2005, (02)

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玉竹中黄酮类成分的薄层色谱指纹图谱研究
Studies on TLC fingerprint of flavonoids in rhizome of Polygonatum odoratum

杨先国,陈四保,陈士林,杨大坚,刘塔斯
YANG Xian-guo~(1,2), CHEN Si-bao~1, CHEN Shi-lin~1, YANG Da-jian~1, LIU Ta-si~2(1. Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hong Kong, China;2. Hunan College of Traditional Chinese Medicine, Changsha 410007, China)

摘要(Abstract):

目的 :对玉竹的黄酮类成分进行薄层色谱指纹图谱研究 ,以期解决玉竹的专属性鉴别 ,并为其质量控制提供依据。方法 :采用薄层色谱法 ,甲苯 醋酸乙酯 甲酸 (5∶4∶1)为展开剂 ,氨水熏蒸显色 ,用λR=5 0 0nm ,λS=2 80nm双波长扫描。结果 :建立了由 10个特征荧光斑点构成的玉竹黄酮类成分的薄层指纹图谱。结论 :该方法可以有效地鉴别玉竹 ,并评价其质量
Objective: To study the thin layer chromatographic (TLC) fingerprint of flavonoid constituents from Polygonatum odoratum, to set up the identification protocol of the herbal and provide scientific information for its quality control. Method: The ethanol extracts were separated on silica gel G precoated plate with a mixture of toluene-ethylacetate-formic acid(5∶4∶1) as the mobile phase. The spots were visualized with ammonia vapor, then were examined under ultravilet light (365 nm). The plate was scanned at wavelengths of λ_R=500 nm,(λ_S=280 nm). Result: A fingerprint of flavonoids of P. odoratum, with 10 specific fluorescent spots while examined under ultraviolet light, was set up. Conclusion: The method can be used for quality control of P. odoratum.

关键词(KeyWords): 玉竹;薄层色谱;指纹图谱
Polygonatum odoratum; TLC; fingerprint.

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作者(Author): 杨先国,陈四保,陈士林,杨大坚,刘塔斯
YANG Xian-guo~(1,2), CHEN Si-bao~1, CHEN Shi-lin~1, YANG Da-jian~1, LIU Ta-si~2(1. Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hong Kong, China;2. Hunan College of Traditional Chinese Medicine, Changsha 410007, China)

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