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2007, (23) 2492-2493

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清风痛胶囊液相色谱指纹图谱测定条件的研究
Determination of HPLC fingerprint of Qingfengtong capsule

张永欣;杨华;张启伟;易红;陈贵品;
ZHANG Yong-xin1,YANG Hua1,ZHANG QI-wei1,YI Hong1,CHEN Gui-pin2(1.Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China;2.Beijing Zichenxuan Pharmaceutical Deal Co.Ltd.,Beijing 100075,China)

摘要(Abstract):

目的:建立清风痛胶囊的色谱指纹图谱测定方法。方法:采用高效液相色谱梯度洗脱法,色谱柱为Diamonsil C18柱(4.6 mm×250 mm,5μm);流动相A0.1 mol.L-1磷酸二氢钾,流动相B甲醇,线性梯度洗脱:0~2min,10%B;2~42 min;10%~90%B。流速1 mL.min-1;检测波长262 nm。结果:在上述色谱条件下测定的色谱指纹图谱各色谱峰分离较好,方法学考察基本达到指纹图谱要求。结论:所建指纹图谱测定条件可用于产品的检测,但用于质量控制尚需作进一步的工作。
Objective:To establish a fingerprint analysis method of Qingfengtong capsule by HPLC.Method: The samples were extracted with 70% ethanol in an ultrasonic bath.The extracts were analyzed at 35 ℃ on a Diamonsil C18 column(4.6 mm×250 mm,5 μm) with 0.1 mol·L-1 potassium dihydrogen phosphate water-solution as mobile phase A,and methanol as mobile phase B.The analysis followed a linear gradient elution program.Initial condition of the mobile phase was 10% B for 2 minutes;then changed to 90% B in 40 minutes.The flow rate was kept at 1.0 ml·min-1 and the detector wavelength was 262 nm.Result: The main peaks in fingerprint chromatogram of Qingfengtong capsules were separated fairly well.The results of method validation meet the requirements for the fingerprints.Conclusion: The established method can be used for the quality control of Qingfengtong capsules.

关键词(KeyWords): 清风痛胶囊;指纹图谱;HPLC
Qingfengtong capsule;chromatograplic fingerprints;HPLC

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作者(Author): 张永欣;杨华;张启伟;易红;陈贵品;
ZHANG Yong-xin1,YANG Hua1,ZHANG QI-wei1,YI Hong1,CHEN Gui-pin2(1.Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China;2.Beijing Zichenxuan Pharmaceutical Deal Co.Ltd.,Beijing 100075,China)

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