中国中药杂志

2020, v.45(21) 5169-5176

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川佛手不同发育时期的比较转录组学分析
Comparative transcriptomics analysis of Citrus medica var. sarcodactylis at different developmental stages

潘媛;陈大霞;李隆云;
PAN Yuan;CHEN Da-xia;LI Long-yun;Chongqing Engineering Research Center for Fine Variety Breeding Techniques of Chinese Materia Medica,Chongqing Sub-center of National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Chongqing Academy of Chinese Materia Medica;

摘要(Abstract):

为研究川佛手不同发育时期转录水平差异,探究调控川佛手有效成分生物合成的基因。该研究利用Illumina Hiseq 4000高通量测序技术对不同发育时期的川佛手果实进行转录组测序,获得121 235条unigene,平均长度2 434 bp,利用DESeq筛选获得3 379个差异基因,主要集中在信号传导、生物调控、代谢过程等生物过程,富集到淀粉、蔗糖的代谢、苯丙素的生物合成、类黄酮的生物合成等代谢通路。进一步对川佛手有效成分生物合成相关基因进行动态比较:PAL,CHI,CYP75B1,ZDS,4CL,FLS的随着果实由绿变黄表达量呈逐渐上升趋势;COMT,F3H,CYP73A的表达量呈先升高后降低趋势;CCR,HCT,HRP呈先下调后上调表达。该研究为进一步挖掘川佛手有效成分生物合成过程中的关键基因,解析调控其有效成分生物合成途径提供研究基础。
In order to study the transcriptional differences of Citrus medica var. sarcodactylis at different developmental stages, we explored the genes regulating the biosynthesis of the effective components. In this study, Illumina Hiseq 4 000 high-throughput sequencing technology was used to sequence the transcriptome of C. medica var. sarcodactylis at different developmental stages, 121 235 unigenes were obtained with an average length of 2 434 bp, 3 379 different genes were obtained using DESeq screening, which mainly connected to biological processes such as signal transmission, biological regulation, and metabolic processes, and enriched in metabolic pathways such as starch, sucrose metabolism, phenylpropanoid biosynthesis, and flavonoid biosynthesis. Further dynamic comparison of biosynthesis related genes of active ingredients: the expression levels of PAL, CHI, CYP75B1, ZDS, 4CL and FLS gradually increased as the fruit turned from green to yellow; the expressions of COMT, F3H and CYP73A increased at first and then decreased; CCR, HCT and HRP were down-regulated whereas up-regulated. This study provides references for further excavation of key genes in the biosynthesis of active components, as well as biopathway analysis of active components for C. medica var. sarcodactylis.

关键词(KeyWords): 川佛手;发育时期;有效成分;基因表达
Citrus medica var.sarcodactylis;development period;active ingredients;gene expression

Abstract:

Keywords:

基金项目(Foundation): 重庆市脱贫攻坚专家团项目(渝人社办[2019]297号);; 现代农业产业技术体系建设专项(CARS-21)

作者(Author): 潘媛;陈大霞;李隆云;
PAN Yuan;CHEN Da-xia;LI Long-yun;Chongqing Engineering Research Center for Fine Variety Breeding Techniques of Chinese Materia Medica,Chongqing Sub-center of National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Chongqing Academy of Chinese Materia Medica;

Email:

DOI: 10.19540/j.cnki.cjcmm.20200722.101

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