中国中药杂志

2008, (22) 2650-2652

[打印本页] [关闭]
本期目录(Current Issue) | 过刊浏览(Past Issue) | 高级检索(Advanced Search)

甘草药材的快速HPLC指纹图谱分析
Fingerprint analysis of Radix Glycyrrhizae by fast HPLC

濮润;王卫星;王京辉;陈有根;傅欣彤;郭洪祝;
PU Run1,WANG Wei-xing1,WANG Jing-hui2,CHEN You-gen2,FU Xin-tong2,GUO Hong-zhu1,2(1.School of Pharmaceutical Sciences,Peking University,Beijing 100083,China;2.Beijing Institute for Drug Control,Beijing 100035,China)

摘要(Abstract):

目的:研究不同地区甘草药材的快速HPLC指纹图谱,为甘草商品药材的鉴别及质量控制提供依据。方法:选择Agilent Eclipse plus C18(4.6 mm×50 mm,1.8μm)快速高效液相色谱柱,乙腈-0.05%磷酸水溶液为流动相梯度洗脱,流速1.0 mL.min-1,记录11 min色谱图。结果:用快速液相色谱方法将甘草药材分析时间从36min缩短为11 min;经过聚类分析将70个产地甘草药材分为胀果甘草和乌拉尔甘草两类,其中59个为乌拉尔甘草,11个为胀果甘草;发现甘草素葡萄糖芹糖苷、甘草苷和甘草酸3个指标成分联合可有效评价甘草品种。结论:甘草药材快速HPLC指纹图谱的专属性强,且加快了甘草药材的分析效率,可用于甘草药材的鉴别及质量控制。我国大多数地区销售的甘草商品药材为乌拉尔甘草,少部分为胀果甘草。
The objective of this paper is to develop a fast analysis method to determine fingerprints of Radix Glycyrrhizae from different areas of China for identification and quality control.The experiments were carried out under following conditions: Agilent Eclipse Plus C18(4.6 mm×50 mm,1.8 μm) column,acetonitrile and 0.05% phosphoric acid solution as the mobile phases with gradient elution,flow rate 1.0 mL·min-1,analysis time 11 min.The run time of the method was obviously decreased from 36 minutes to 11 minutes compared with routine HPLC method.The cluster analyses of the fingerprints of the 70 samples were performed by SPSS.The results showed that all samples were classified into 2 groups,59 Glycyrrhiza uralensis as well as 11 G.inflata.Three compounds,liquiritin apioside,liquiritin and glycyrrhiza acid should be considered as effective references for quality control of Radix Glycyrrhizae.This method can be used widely for identification and quality control of Radix Glycyrrhizae.

关键词(KeyWords): 甘草;快速HPLC;指纹图谱
Radix Glycyrrhizae;fingerprint;fast HPLC

Abstract:

Keywords:

基金项目(Foundation): 北京市自然科学基金课题(7062039)

作者(Author): 濮润;王卫星;王京辉;陈有根;傅欣彤;郭洪祝;
PU Run1,WANG Wei-xing1,WANG Jing-hui2,CHEN You-gen2,FU Xin-tong2,GUO Hong-zhu1,2(1.School of Pharmaceutical Sciences,Peking University,Beijing 100083,China;2.Beijing Institute for Drug Control,Beijing 100035,China)

Email:

DOI:

扩展功能
本文信息
服务与反馈
本文关键词相关文章
本文作者相关文章
中国知网
分享