中国中药杂志

2003, (04)

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三七HPLC指纹图谱的建立
Fingerprint Construction of Panax notoginseng

王雁,毕开顺
WANG Yan 1, BI Kai shun 2(1. Panjin Institute of Drug Control,Panjin 124010, Liaoning, China; 2.Shenyang Pharmaceutical University,Shenyang 110015, Liaoning, China)

摘要(Abstract):

目的 :建立三七药材的高效液相指纹图谱 (HPLC FP) ,在量上为三七的鉴定与合理应用提供依据。方法 :LunaC1 8(2 )色谱柱 ,流动相 :(A)乙腈 (B) 0 .0 5 %磷酸梯度洗脱 ,0~ 8min(2 0 %A ,80 %B) ,8~ 2 0min(2 0 %A~ 40 %A ,80 %B~ 60 %B) ,2 0~ 30min(40 %A~ 2 0 %A ,60 %B~ 80 %B) ,30~ 36min(2 0 %A~ 1 0 0 %A ,80 %B~0 ) ,36~ 50min (1 0 0 %A) ,50~ 65min(1 0 0 %A~ 2 0 %A ,0~ 80 %B) ,流速 1 .0mL·min- 1 ,检测波长 2 0 3nm。咖啡因为内标物。结果 :通过聚类分析 ,2 1个不同产地、不同规格的三七样品被聚为 4类 :地道药材优级品、地道药材、市售一般品和次品。通过相似度计算 ,2 1个三七样品的相似度均在 0 .9~ 1 .0之间 ,8个常见伪品的相似度均小于0 .9。结论 :该色谱图可作为三七的HPLC FP ,用于三七的真伪鉴别和质量评价
Objective: To provide application and identification basis for Panax notoginseng by constructing the HPLC FP of Panax Notoginsing . Method: The luna C 18 (2) column was used with a mobile phase of (A)acetonitrile (B)0.05%phosphoric acid gradient elution, 0~8 min (20%A,80%B), 8~20 min (20%A~40%A,80%B~60%B),20~30 min(40%A~20%A,60%B~80%B),30~36 min(20%A~100%A,80%B~0),36~50 min(100%A),50~65 min (100%A~20%A,0~80%B). The flow rate was 1.0 ml·min -1 .The wavelength of detecter was set at 203 nm. Caffeine was internal standard. Result:By Cluster Analysis, the twenty one notoginseng samples were classified as four clusters:the superior in producing area,the ordinary in producing area ,the ordinary and the inferior .By Similarty Calculation ,the similarity of the twenty one notoginseng samples were 0.9 1.0, and the similarity of the eight common falses are less than 0.9. Conclusion: The HPLC FP of notoginseng has been established.The method can be used to identify and evaluate the quality of notoginseng.[

关键词(KeyWords): 三七;指纹图谱
Panax notoginseng ; HPLC FP

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作者(Author): 王雁,毕开顺
WANG Yan 1, BI Kai shun 2(1. Panjin Institute of Drug Control,Panjin 124010, Liaoning, China; 2.Shenyang Pharmaceutical University,Shenyang 110015, Liaoning, China)

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