中国中药杂志

2010, v.35(02) 183-186

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不同产地川党参药材的HPLC指纹图谱
HPLC fingerprint of Codonopsis tangshen from different habitats

彭锐;马鹏;李隆云;卫莹芳;
PENG Rui1,2,MA Peng1,LI Longyun1,WEI Yingfang2(1.Chongqing Engineering Research Center for Fine Variety Breeding Techniques of Chinese Materia MedicaChongqing Academy of Chinese Materia Medica,Chongqing 400065,China;2.Chengdu University of TraditionalChinese Medicine,Chengdu 610075,China)

摘要(Abstract):

目的:建立川党参的高效液相色谱指纹图谱,为科学评价和有效控制其质量提供可靠的方法。方法:用HPLC-DAD方法测定了29产地川党参药材样品,采用药典委员会颁布的中药色谱指纹图谱相似度评价系统2004 A进行评价,建立了共有模式,以相关度评价图谱的相似性,以共有峰与对照峰的比值对各产地川党参药材进行聚类分析。结果:29产地川党参药材的色谱指纹图谱有7个共有峰,高效液相色谱指纹图谱的相似度都在0.8以上,23产地川党参药材可通过系统聚类归为一类,不同产地川党参质量相似性较好。结论:本方法可用于川党参药材质量评价。
Objective: To establish a sensitive and specific HPLC method for quality control of Codonopsis tangshen.Method:The samples were determined with the dereloped method.By the Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine(Version 2004A),mean chromatogram was generated as the representative standard fingerprint and the similarity of each chromatogram against the mean chromatogram was also calculated.Samples was clustered using principal component analysis(PCA) based on the ratio of characteristic peaks and standard peak.The chromatographic fingerprinting of C.tangshen,showing 7 characteristic peaks,was established from 29 habitats of C.tangshen.Result: The similarity of the chromatographic fingerprints from the 29 samples was over 0.8 and 23 samples were classified into one group based on hierarchical cluster,which showed the quality of C.tangshen.from different habitats was in good consistency.Conclusion: The method can be applied for quality assessment of C.tangshen.

关键词(KeyWords): 川党参;产地;HPLC;指纹图谱
Codonopsis tangshen;habitats;HPLC;chromatographic fingerprint

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基金项目(Foundation): 国家“十一五”科技支撑计划项目(2006BAI06A11-9)

作者(Author): 彭锐;马鹏;李隆云;卫莹芳;
PENG Rui1,2,MA Peng1,LI Longyun1,WEI Yingfang2(1.Chongqing Engineering Research Center for Fine Variety Breeding Techniques of Chinese Materia MedicaChongqing Academy of Chinese Materia Medica,Chongqing 400065,China;2.Chengdu University of TraditionalChinese Medicine,Chengdu 610075,China)

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