中国中药杂志

2015, v.40(12) 2314-2317

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铁皮石斛叶片多糖和醇溶性浸出物动态积累规律研究
Study on accumulation of polysaccharides and alcohol-soluble extracts contents of Dendrobium officinale leaves

刘振鹏;徐翠霞;刘京晶;斯金平;张新凤;吴令上;
LIU Zhen-peng;XU Cui-xia;LIU Jing-jing;SI Jin-ping;ZHANG Xin-feng;WU Ling-shang;Nurturing Station for State Key Laboratory of Subtropical Silviculture,Zhejiang Agricultural and Forestry University;Zhejiang Forestry Academy;

摘要(Abstract):

该文通过揭示铁皮石斛叶片多糖和醇溶性浸出物含量的动态变化规律,为铁皮石斛叶片资源的开发利用提供依据。采用苯酚-硫酸法和醇溶性浸出物热浸法测定铁皮石斛3个优良品系不同月份的叶片中多糖和浸出物含量。结果表明,铁皮石斛叶片多糖质量分数为4.45%~12.17%,约为茎的1/4,醇溶性浸出物质量分数为7.45%~29.34%,约为茎的1.5倍。铁皮石斛叶片多糖含量变化与物候期密切相关,冬季、萌芽初期和落叶期植株代谢水平降低,叶片多糖含量减少;植株生长旺盛期,叶片多糖含量较高。叶片中浸出物含量在萌芽前达到最高值,促进了新芽的萌动。
This paper revealed the accumulation regularity of polysaccharides and alcohol-soluble extracts contents of Dendrobium officinale leaves,which have provided basis for the development and utilization of the leaves. The polysaccharides and alcohol-soluble extracts contents of three D. officinale strains leaves collected in different growing periods were determined by phenol-sulfuric acid method and hot-dip method respectively. The results showed that the content of polysaccharides in leaves was 4. 45%-12. 17%,and was about a quarter in stems. The alcohol-soluble extracts content in leaves was 7. 45%-29. 34%,and was 1. 5 times that of stems.The quality variation of polysaccharides in leaves was closely related to the phenophase. The leaves with lower level of metabolism in three stages: winter,early germination stage and deciduous period,which led to lower content of polysaccharides. The leaves at the vigorous growth stage with higher content of polysaccharides. The alcohol-soluble extracts were closely associated to the formation and germination of buds. The content of alcohol-soluble extracts peaked before sprout,and promoted the growth of new shoots.

关键词(KeyWords): 铁皮石斛;叶片;多糖;浸出物;品系;采收期
Dendrobium officinale;leaf;polysaccharides;alcohol-soluble extract;strain;harvesting time

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基金项目(Foundation): 浙江省自然科学基金项目(LY14C020005);; 浙江省产业技术创新战略联盟项目(2012LM201)

作者(Author): 刘振鹏;徐翠霞;刘京晶;斯金平;张新凤;吴令上;
LIU Zhen-peng;XU Cui-xia;LIU Jing-jing;SI Jin-ping;ZHANG Xin-feng;WU Ling-shang;Nurturing Station for State Key Laboratory of Subtropical Silviculture,Zhejiang Agricultural and Forestry University;Zhejiang Forestry Academy;

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