中国中药杂志

2017, v.42(21) 4172-4179

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怀地黄块根中菊花心与非菊花心部位的化学质量特征比较
Comparison of chemical quality characteristics between radial striations and non-radial striations in tuberous root of Rehmannia glutinosa

谢彩侠;张苗;李雅静;耿晓桐;王丰青;张重义;
XIE Cai-xia;ZHANG Miao;LI Ya-jing;GENG Xiao-tong;WANG Feng-qing;ZHANG Zhong-yi;Henan University of Traditional Chinese Medicine;Fujian Agriculture and Forestry University;Henan Agricultural University;

摘要(Abstract):

利用HPLC对怀地黄"北京1号"生育期内块根中菊花心、非菊花心及整体块根三部位中梓醇、毛蕊花糖苷、地黄苷A、地黄苷D、益母草苷进行含量测定,并结合其HPLC指纹图谱进行整体化学质量特征比较。结果表明,生育期内怀地黄主要成分含量变化趋势不同;各成分在菊花心与非菊花心部位富集量差别较大。梓醇、毛蕊花糖苷与地黄苷D在菊花心与非菊花心中的分布相对均匀,非菊花心部位地黄苷A的含量明显高于菊花心部位,而菊花心部位益母草苷的含量则明显高于非菊花心部位;地黄菊花心、非菊花心及整体块根3部位的HPLC指纹图谱色谱行为基本一致,但是化学成分含量相差较大;通过聚类分析,怀地黄菊花心部位与非菊花心部位、整体块根明显被分为2类。因此,怀地黄菊花心与非菊花心部位化学质量特征存在明显差异。该研究为怀地黄的质量评价和道地性的揭示提供依据。
An HPLC method was established to determine the contents of catalpol,acteoside,rehmaionoside A,rehmaionoside D,leonuride in three part of Rehmanni glutinosa in Beijing No. 1 variety R. glutinosa during the growth period,This method,in combination with its HPLC fingerprint was used to evaluate its overall quality characteristics. The results showed that:(1) the content of main components of R. glutinosa varied in different growth stages;(2) there was a great difference of the content of main components between theradial striations and the non-radial striations;(3) the two sections almost have the same content distribution of catalpol,acteoside and rehmaionoside D;(4)the content of rehmaionoside A in non-radial striations was higher than that in radial striations,while the content of leonuride in radial striations was higher than that in non-radial striations.;(5)the HPLC fingerprint of radial striations,non-radial striations and whole root tuber were basically identical,except for the big difference in the content of chemical components. The result of clustering displayed that the radial striations,non-radial striations,and whole root were divided into two groups. In conclusion,there was a significant difference in the quality characteristics of radial striations and non-radial striations of R. glutinosa. This research provides a reference for quality evaluation and geoherbalism of R. glutinosa.

关键词(KeyWords): 菊花心;化学成分;道地性;HPLC指纹图谱;主成分分析;聚类分析
radial striations;chemical composition;geoherbalism;HPLC fingerprints;PCA;cluster analysis

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(81473299);; 河南省自然科学基金项目(162300410157)

作者(Author): 谢彩侠;张苗;李雅静;耿晓桐;王丰青;张重义;
XIE Cai-xia;ZHANG Miao;LI Ya-jing;GENG Xiao-tong;WANG Feng-qing;ZHANG Zhong-yi;Henan University of Traditional Chinese Medicine;Fujian Agriculture and Forestry University;Henan Agricultural University;

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DOI: 10.19540/j.cnki.cjcmm.20170905.004

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