中国中药杂志

2016, v.41(22) 4144-4149

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紫杉醇生物合成途径及合成生物学研究进展
Recent advances in biosynthetic pathway and synthetic biology of taxol

匡雪君;王彩霞;邹丽秋;李滢;孙超;
KUANG Xue-jun;WANG Cai-xia;ZOU Li-qiu;LI Ying;SUN Chao;Institute of Medicinal Plant Development,China Academy of Medical Sciences and Peking Union Medical College;Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences;

摘要(Abstract):

紫杉醇是一种从红豆杉植物中提取的具有显著抗癌效果的萜类次生代谢产物。作为有效的抗癌药物,目前生产主要依赖于红豆杉,供求矛盾十分突出。近年来,利用合成生物学技术建立新的紫杉醇来源途径已成为研究热点。目前,紫杉醇合成途径基本框架已经确定;参与紫杉醇合成相关酶基因大部分已被克隆和鉴定;已经在大肠杆菌和酿酒酵母中异源合成了紫杉醇的前体物质紫杉烯和5α-羟基紫杉烯。该研究对紫杉醇生物合成途径及紫杉醇药物中间体在大肠杆菌和酿酒酵母工程细胞中的合成进展进行了综述,以期为生物合成紫杉醇进一步研究提供参考。
Taxol,a kind of terpenoid secondary metabolite produced by Taxus brevifolia,is an effective anticancer drug that manufacture relies mainly on the extraction form plants. In order to solve the resource shortage,a lot of work has been done to develop the alternative method. Recently,using synthetic biology to realize heterologous biosynthesis of the precursors of taxol has become a hotspot. Now,the basic framework of taxol biosynthetic pathways has been confirmed,and most enzyme genes involved in taxol biosynthesis have been cloned and identified. The two taxol precursors,taxa-4( 5),11( 12)-diene and taxa-4( 20),11( 12)-dien-5α-ol,have been synthesized in Escherichia coli and Saccharomyces cerevisiae. Here this paper reviewed the recent advances in the biosynthetic pathway of taxol and the latest developments of synthetic biology,which aims to provide a guidance for the heterologous biosynthesis of taxol.

关键词(KeyWords): 紫杉醇;合成途径;合成生物学
taxol;biosynthetic pathway;synthetic biology

Abstract:

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基金项目(Foundation): 国家自然科学基金面上项目(81273485,81573704)

作者(Author): 匡雪君;王彩霞;邹丽秋;李滢;孙超;
KUANG Xue-jun;WANG Cai-xia;ZOU Li-qiu;LI Ying;SUN Chao;Institute of Medicinal Plant Development,China Academy of Medical Sciences and Peking Union Medical College;Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences;

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