中国中药杂志

2004, (02)

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RP-HPLC测定川芎挥发油中藁本内酯的含量
Determination of ligustilide in volatile Oil from rhizome of Ligusticum Chuanxiong by RP-HPLC

林燕芝,唐星,毕开顺
LIN Yan-zhi,TANG Xing,BI Kai-shun ( School of Pharmacy,Shenyang Pharmaceutical University,Shenyang 110016,China )

摘要(Abstract):

目的 :测定川芎挥发油中藁本内酯的含量。方法 :采用RP HPLC法 ,内标法定量 ,内标物尼群地平 ,HypersilODS2 色谱柱 (4.6mm× 2 0 0mm ,5 μm) ,流动相为甲醇 乙腈 水 (33∶2 1∶4 6 ) ,检测波长 2 75nm。 结果 :藁本内酯在 2 .92~ 2 9.2mg·L-1线性关系良好 ,藁本内酯在川芎挥发油中的含量为 34.79% ,藁本内酯平均回收率 95 .1% ,RSD为 2 .3%。结论 :本方法简便 ,准确 ,重现性好 ,可用于藁本内酯的含量测定。
Objective: To determine Ligustilide in volatile oil from Ligustrcum chuanxiong with RP-HPLC. Method: ODS 2 column (4.6 mm×200 mm,5 μm)was used and nitrendipine was used as internal standard. The mobil phase consisted methanol,acetontrile and water(33∶21∶46). The ligustilide was at 275 nm. Result: The linear range was 2.92~29.2 mg·L -1 for ligustilide. The average recovery of ligustilide was 95.1% and RSD was 2.3%. Conclusion: This method is simple and can be used to determine ligustilide with satisfactory accuracy and reproducibility.

关键词(KeyWords): 川芎;挥发油;藁本内酯;RP-HPLC
Ligusticum chuanxiong ;volatile oil;ligustilide;RP-HPLC

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作者(Author): 林燕芝,唐星,毕开顺
LIN Yan-zhi,TANG Xing,BI Kai-shun ( School of Pharmacy,Shenyang Pharmaceutical University,Shenyang 110016,China )

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