中国中药杂志

2014, v.39(14) 2699-2703

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木香川木香药材UPLC特征指纹图谱建立及鉴别研究
UPLC fingerprint spectra for discrimination of Aucklandiae Radix and Vladimiriae Radix

吕露阳;张吉仲;张志锋;刘圆;曾锐;鲁建美;任焕明;
LV Lu-yang;ZHANG Ji-zhong;ZHANG Zhi-feng;LIU Yuan;ZENG Rui;LU Jian-mei;REN Huan-ming;Ethnic Medicine Institute,Southwest University for Nationalities;

摘要(Abstract):

木香与川木香成分相似,难以鉴别。该文采用ACQUITY UPLC色谱仪,乙腈-0.05%磷酸水溶液梯度洗脱,检测波长238 nm,建立了木香、川木香药材的UPLC指纹图谱。聚类分析和主成分分析可以完全将2种药材分开。指纹图谱分析木香药材中标定了8个共有峰;川木香药材中标定了12个共有峰。通过与对照品比对,指认了其中6个共有峰,分别为紫丁香苷、绿原酸、异绿原酸A、异绿原酸B、木香烃内酯和去氢木香内酯。川木香中的4个特征成分峰,在木香样品中并不具有。应用UPLC特征指纹图谱对木香和川木香进行鉴别是可行的。
It's difficult to identify Aucklandiae Radix and Vladimiriae Radix because of their similar composition. In this paper,UPLC method was used to establish their UPLC fingerprint to identify them with the mobile of acetonitrile-0. 05% phosphoric acid water solution by gradient elution at the detection wavelength of 238 nm. Clustering analysis and principal components analysis showed that Vladimiriae Radix was significantly different from Aucklandiae Radix. Eight common peaks and twelve common peaks were defined respectively in Aucklandiae Radix and Vladimiriae Radix herbs by fingerprint analysis. Six of them were identified as syringoside,chlorogenic acid,isochlorogenic acid A,isochlorogenic acid B,costunolide and dehydrocostuslactone by comparing with standard references. There are four peaks in all of Vladimiriae Radix samples and in none of Aucklandiae Radix samples. So UPLC fingerprint can be used to identify these two herbs.

关键词(KeyWords): 木香;川木香;指纹图谱;UPLC;鉴别
Aucklandiae Radix;Vladimiriae Radix;fingerprint;UPLC;identification

Abstract:

Keywords:

基金项目(Foundation): 国家科技支撑计划项目(2012BAI27B07);; 四川省科技厅应用基础项目(2013YJ0042);; 国家外专局外国文教专家项目(2014-084);; 四川省2011协同创新中心“青藏高原生态畜牧业协同创新中心”项目

作者(Author): 吕露阳;张吉仲;张志锋;刘圆;曾锐;鲁建美;任焕明;
LV Lu-yang;ZHANG Ji-zhong;ZHANG Zhi-feng;LIU Yuan;ZENG Rui;LU Jian-mei;REN Huan-ming;Ethnic Medicine Institute,Southwest University for Nationalities;

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