中国中药杂志

2007, (11) 1057-1060

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复方中药对裸鼠肝癌模型PTEN蛋白表达的影响
Expression of PTEN in athymic mice with HCC treated by complex prescription of Chinese crude drug

孙保国;陈泽雄;张诗军;刘泳冬;黄红中;尹丽荣;
SUN Bao-guo CHEN Ze-xiong ZHANG Shi-jun LIU Yong-dong HUANG Hong-zhong YIN Li-rong (The First Affiliated Hospital of Sun Yat-Sen university,Guangzhou 510080,China)

摘要(Abstract):

目的:探讨中药复方对间接原位移植肝癌裸鼠模型的干预作用。方法:采用人肝细胞癌细胞株Bel- 7402间接原位移植,造雄性肝癌裸鼠模型48只,造模后24 h随机分4组即中药复方低、中、高3个剂量组和FT207化疗组,4周后处死裸鼠,观察正常肝组织、肝癌旁组织、肝癌组织的组织形态情况,并用二步法免疫组织化学检测肝组织、肝癌旁组织、肝癌组织中PTEN蛋白的表达。结果:造模解剖证实获得100%的成功率,中位生存时间24 d (12~28d);瘤鼠肝脏PTEN蛋白表达强度癌旁组织最高,癌组织最低,正常肝组织次之(P<0.01)。中药治疗相对于FT207化疗而言可明显增加PTEN蛋白的表达强度,中药组组间比较提示中剂量组效果最好(P<0.05或P<0.01),低剂量和高剂量差别不大。结论:癌旁组织中PTEN的高表达,有可能是机体的应激保护反应之一;适宜的中药浓度与抗癌效果有关;本方治疗肝癌的部分机制可能是通过增强PTEN蛋白表达来实现的。
Objective:To research the treatment effect of complex prescription of Chinese crude drug in BALB/c athymic mice with human liver cancer,which were built by Bel-7402.Method:48 male BALB/c athymic mouse models were built by Bel-7402 with an indirect method.After 24 hours of postoperation,the 48 athymic mice were distributed randomly into 4 groups which were treated by intragastric administration with complex prescription of Chinese crude drug that had been deliquated into 3 groups by the different densi- ty as the low,middle,and high and FT207(Tegafur)for4 weeks.At last,athymic mice were put to death and PTEN was detected in hepatic tissue,latero-cancer tissue and cancer tissue by immunohistochemistry(Power Vision~(TM) Two-Step Histostaining Reagent).Re- sult:All of the 48 athymic mice survived 12 to 28 days(Ms 24 days)and every mouse with liver cancer demonstrated by dissection. The result of immunohistochemistry represents that the intensity of PTEN in latero-cancer tissue is the highest,and then the hepatic tis- sue,the lowest is cancer tissue,P<0.01.It also represents that the intensity of PTEN in treatment groups(A,B,C)is more higher than the control group(D),P<0.05 or P<0.01,and group B is the highest in the treatment groups,P<0.05 or P<0.01.Howev- er,there is no significant statistic difference between group A and group C.Conclusion:The higher expression of PTEN in the latero- cancer tissue can represent the protective reaction of stress of the organism.And anticancer effect of this complex prescription of Chi- nese crude drug relates to an eligible density of it.Mechanisms of this complex prescription of Chinese crude drug healing HCC may partially be explained by enhancing the expression of PTEN in liver.

关键词(KeyWords): 裸鼠肝癌模型;复方中药;PTEN
complex prescription of Chinese crude drug;HCC-model of athymic mouse;PTEN

Abstract:

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基金项目(Foundation): 广东省科技计划项目(2005833001040);; 广东省中医药局资助项目(1040056,301014)

作者(Author): 孙保国;陈泽雄;张诗军;刘泳冬;黄红中;尹丽荣;
SUN Bao-guo CHEN Ze-xiong ZHANG Shi-jun LIU Yong-dong HUANG Hong-zhong YIN Li-rong (The First Affiliated Hospital of Sun Yat-Sen university,Guangzhou 510080,China)

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