中国中药杂志

2008, (16) 1968-1971

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罗布麻总黄酮高效液相色谱指纹图谱研究
HPLC fingerprint of total flavonoids of Folium Apocyni Veneti

郝旭亮;张素琼;王晓剑;李青山;
HAO Xu-liang,ZHANG Su-qiong,WANG Xiao-jian,LI Qing-shan(School of Pharmaceutical Science,Shanxi Medical University,Taiyuan 030001,China)

摘要(Abstract):

目的:建立罗布麻总黄酮有效部位的指纹图谱,为科学评价及有效控制其质量提供可靠方法。方法:采用HPLC-DAD法进行测定,色谱柱:Kromasil C18(4.6 mm×250 mm,5μm),流动相0.05%磷酸乙腈-0.05%磷酸水梯度洗脱,柱温25℃,流速1.0 mL.min-1;检测波长360 nm。结果:测定7批罗布麻总黄酮的HPLC图,建立标准指纹图谱,有17个共有峰,并用中药指纹图谱相似度评价系统计算其相似度,各批总黄酮的相似度介于0.95~1.0。梯度洗脱得到的色谱图色谱蜂分离较好,达到了指纹图谱的要求,并在相同的色谱条件下测定了罗布麻叶药材的HPLC图谱,其结果与罗布麻总黄酮有很好的相关性。结论:该方法精密度、稳定性及重现性较好,特征性及专属性强,可全面控制罗布麻总黄酮及其制剂质量,确保每批产品的均一性。
Objective: To establish a sensitive and specific HPLC fingerprint for the quality controlling of total flavonoids of Folium Apocyni Veneti.Method: HPLC analysis was performed on a Kromasil C18 column(4.6 mm×250 mm,5 μm) with the mixture of solvent A [acetonitrile-phosphoric acid(95∶5)] and solvent B(0.05% phosphoric acid) in gradient mode at a flow rate of 1.0 mL·min-1.The detection wavelength was set at 360 nm.The column temperature was set at 25 ℃ and the injection volume was 20 μL.Result: The chromatographic fingerprint of total flavonoids was established which showed 17 characteristic peaks from 7 patches of total flavonoids products.The similarity from different patches was 0.95-1.00 analyzed by the software of 'Computer-aided Similarity Evaluation' and showed high similitude in peak numbers and the retention time.Moreover,comparison of the HPLC profiles of the total flavonoids with the corresponding Folium Apocyni Veneti leaves indicated that they were closely related to each other.Conclusion: The chromatographic fingerprint of the total flavonoids with high specificity and can be used to control its quality and assure the homogenicity for each patch of the total flavonoids.

关键词(KeyWords): 罗布麻叶;总黄酮;高效液相色谱;指纹图谱
Folium Apocyni Veneti;total flavonoids;HPLC;fingerprint

Abstract:

Keywords:

基金项目(Foundation): 山西省自然科学基金(20031102);; 山西省教育厅攻关课题(51250001-2023);; 太原市大学生创新创业扶持项目(07010780)

作者(Author): 郝旭亮;张素琼;王晓剑;李青山;
HAO Xu-liang,ZHANG Su-qiong,WANG Xiao-jian,LI Qing-shan(School of Pharmaceutical Science,Shanxi Medical University,Taiyuan 030001,China)

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