中国中药杂志

2006, (24) 2033-2035

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天花粉药材及其类似品的分子鉴别新方法
A new molecular method to authenticate Radix Trichosanthis as well as its adulterants and substitutes

崔光红;黄璐琦;王敏;
CUI Guang-hong HUANG Lu-Qi WANG Min (Institute of Chinese Materia Medica,Academy of Chinese Medical Sciences,Beijing 100700,China)

摘要(Abstract):

目的:寻找天花粉类药材快捷准确的分子鉴别方法。方法:在天花粉ITS保守区域设计3个20 mer左右单引物,对19批天花粉商品及其9种伪品进行PCR扩增。结果:引物TkS1-64得到天花粉及其伪品的DNA多态性图谱,其中560 bp和960 bp条带为天花粉的特征鉴别条带。结论:引物TkS1-64具有稳定性好、重复性高的优点,可以作为天花粉类药材分子鉴别引物。此方法定名为锚定引物扩增多态性DNA(anchored primer amplification polymorphism DNA,简称APAPD),在中药材鉴别中具有推广应用的价值。
Objective:To explore a new molecular method to authenticate Radix Trichosanthis.Method:Three 20 mer prim- ers based on the ITS sequence was designed.The PCR reaction system was optimized and applied to nineteen different sources of Radix Trichosanthis and nine adulterants and substitutes.Result:Polymorphic map of Radix Trichosanthis and its adulterants was obtained from primer TKS1-64.560 bp and 960 bp bands were authentic markers for Radix Trichosanthis.Conclusion:Primer TKSI-64F pos- sesses the advantages of good stability and reproducibility.This new method is named as anchored primer amplification polymorphism DNA(APAPD).It was a potential method to used in molecular identification of other meteria medica.

关键词(KeyWords): 天花粉;类似品;分子鉴别;锚定引物扩增多态性DNA
Radix Trichosanthis;its adulterants and substitutes;molecular identification;APAPD

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作者(Author): 崔光红;黄璐琦;王敏;
CUI Guang-hong HUANG Lu-Qi WANG Min (Institute of Chinese Materia Medica,Academy of Chinese Medical Sciences,Beijing 100700,China)

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