沈玲红,王彬尧,王长谦,谢秀兰,俞国瑞,姚中一,朱元龙,杨葆津
SHEN Ling-hong 1, WANG Bin-yao 1, WANG Chang-qian 1, XIE Xiu -lan 1, YU Guo-rui 2, YAO Zhong-yi 2, ZHU Yuan-long 2, YANG Bao-jin 2 (1. Department of Cadiology, Renji Hospital of Shanghai Second Medi cal University, Shanghai 200001, China; 2. Shanghai Ben-cao Bio-medicine Institute, Shanghai 200040, China) [

• 1:上海第二医科大学附属仁济医院心内科
• 2:上海第二医科大学附属仁济医院心内科
• 3:上海第二医科大学附属仁济医院心内科
• 4:上海第二医科大学附属仁济医院心内科

摘要(Abstract)：

目的 :探讨丹参水溶性部位钙拮抗作用的主要有效成分。方法 :采用荧光探针Fura 2定量分析法 ,分别测定丹参注射液及其活性成分丹参素、原儿茶醛对离体健康成人红细胞胞浆Ca2 + 浓度的影响。结果 :丹参素和原儿茶醛均有降低成人红细胞胞浆Ca2 + 浓度的作用 ,并表现出剂量依赖性 ,二者合并用药 ,药效相加 ,作用与丹参注射液相似。结论 :丹参素和原儿茶醛可能是丹参注射液钙拮抗作用的主要有效成分。
Objective: To study the predominant calcium-an ta gonist components of Danshen injection. Method:The effects of danshensu, protocatechualdehyde and Danshen injection on calcium concentratio n in cytoplasm of erythrocytes were examined in vitro by the fluorescent Ca 2+ -chelator fura-2. Result: Either DS182 or PCAD can d ecrease in dose-dependent cytosolic free calcium concentration in human erythro cytes. They had additive effect when mixed, which was similar to Danshen inje ction. Conclusion: DS182 and PCAD may be predominant calcium- antagonist components of Danshen injection. [

关键词(KeyWords)： 丹参注射液;丹参素;原儿茶醛;钙离子;红细胞
Danshen injection; danshensu; protocatechuald ehyde; Ca 2+ ; erythrocyte

Abstract:

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作者(Author): 沈玲红,王彬尧,王长谦,谢秀兰,俞国瑞,姚中一,朱元龙,杨葆津
SHEN Ling-hong 1, WANG Bin-yao 1, WANG Chang-qian 1, XIE Xiu -lan 1, YU Guo-rui 2, YAO Zhong-yi 2, ZHU Yuan-long 2, YANG Bao-jin 2 (1. Department of Cadiology, Renji Hospital of Shanghai Second Medi cal University, Shanghai 200001, China; 2. Shanghai Ben-cao Bio-medicine Institute, Shanghai 200040, China) [

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参考文献(References)：

• [1]　沈玲红,王彬尧,王长谦丹参注射液对人红细胞胞浆Ca2+浓度的影响中国微循环,2001,5(3):183
• [2]　盛景芬高效液相色谱法测定丹参注射液中的原儿茶醛和丹参素中成药研究,1987,(7):14
• [3]　DavidDM ,MontenayGT ,DevynckMA Fluorescencemeasure mentsoffreeCa2+ concentrationinhumanerythrocytesusingtheCa2+ indicatorfura2CellCalcium,1988,9:167
• [4]　Jinzhong juenAdditionindrugcombinationActaPharmacologicaSinica,1980,1(2):70
• [5]　孙可青,徐长庆,王新一,等丹参素的抗心律失常作用及其电生理机制的研究中国中医药科技,2000,7(3):171
• [6]　徐长庆,李哲泓,王孝铭,等丹参对豚鼠心室乳头肌动作电位的影响哈尔滨医科大学学报,1991,25(6):406
• [7]　VareckaL ,CarafoliE Vanadate inducedmovementsofCa2+ andK+inhumanredbloodcellsJBiolChem,1982,257(13):7414
• [8]　SowemimoCSO ,KovacsIB ,KirbyJDT ,etalEffectsofvera pamiloncalcium inducedrigidityandonfilterabilityofredbloodcellsfromhealthyvolunteersandpatientswithprogressivesystemicsclerosisBrJClinPharmacol,1985,19:731