汪祺;张玉杰;姜昊;孙万晶;
WANG Qi,ZHANG Yu-jie,JIANG Hao,SUN Wan-jing(1.School of Chinese material medica,Beijing University of Chinese Medicine,Bejing 100102,China)

• 1:北京中医药大学中药学院
• 2:北京中医药大学中药学院
• 3:北京中医药大学中药学院
• 4:北京中医药大学中药学院 北京100102

摘要(Abstract)：

目的:比较研究大鼠灌胃双黄连粉针剂及各单味药提取物入血前后主要成分的变化,为优化双黄连粉针剂处方提供实验依据。方法:建立双黄连粉针剂、各单味药提取物及大鼠灌胃后血浆成分的HPLC指纹图谱,分析鉴定双黄连粉针剂及其提取物给药后不同时间入血成分、相对含量、来源及其代谢产物。结果:灌胃双黄连后8个主要成分入血,分别为黄芩苷及其3个代谢产物,绿原酸及其代谢产物和1个金银花中原型化学成分;随着给药时间的延长绿原酸和黄芩苷等原型成分的相对含量逐渐减少,而它们的代谢物逐渐增加;未发现有明显连翘成分入血。结论:入血成分及其代谢产物是双黄连粉针剂的体内药效物质基础,对其进行深入研究,将有助于双黄连粉针剂的处方优化及临床合理用药。
Objective:To investigate the main constituents absorbed in the blood after orally administrating Shuanghuanglian injection extracts of its composed crude drug and provide the foundation for optimizing the prescription of Shuanghuanglian powder for injection.Method:Through analysing HPLC fingerprint established based on shuanghuanglian powder for injection and extracts of it composed crude drug,the active investigation were carried out among the peaks of control plasma and plasma containing drug in rats,shuanghuanglian powder for injection and extract of its composed crude drug.And the analysis was performed about relative content of constituents in each sample.Furthermore their original drugs and the metabolites in plasma containing drug were ascertained.Result:Eight compounds were detected in plasma after oral administration of Shuanghuanglian,four of them were the original compounds contained in Shuanghuanglian powder for injection and three of them were relative metabolites.It has also found that contents of original compounds such as baicalin and chlorogenic acid fell off gradually,but their metabolites increased in rat plasma in vivo.With increasing time the main components of forsythia have not been detected obviously in rats plasma.Conclusion:In some extent,the compounds absorbed into blood from Shuanghuanglian powder for injection and their metabolites are the effective constituents,and its plasma pharmacochemistry should be subject to illuminate the pharmacological effects and the active mechanism.

关键词(KeyWords)： 双黄连粉针剂;入血成分;血浆HPLC指纹图谱
Shuanghuanglian powder for injection;plasma HPLC fingerprint chemical;constituents absorbed in blood

Abstract:

Keywords:

基金项目(Foundation):

作者(Author): 汪祺;张玉杰;姜昊;孙万晶;
WANG Qi,ZHANG Yu-jie,JIANG Hao,SUN Wan-jing(1.School of Chinese material medica,Beijing University of Chinese Medicine,Bejing 100102,China)

Email:

DOI:

参考文献(References)：

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