中国中药杂志

2009, v.34(14) 1758-1760

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海龙及其常见伪品的RAPD鉴别
Identification on Syngnathus and its adulterants with random amplified polymorphic DNA

吴艳;刘佳;王梦月;鞠培俊;李晓波;
WU Yan,LIU Jia,WANG Mengyue,JU Peijun,LI Xiaobo(School of Pharmacy,Shanghai Jiao Tong University,Shanghai 200240,China)

摘要(Abstract):

目的:提供一种鉴别中药材海龙及其伪品的方法。方法:采用随机引物扩增DNA技术,对拟海龙、刁海龙、尖海龙、粗吻海龙、海蠋鱼、宝珈海龙进行基于Nei&Li's相似系数的UPGMA聚类分析,并构建其鉴别模式。结果:从18条随机引物中筛选到LJ04,LJ09,LJ16,LJ19 4条引物能扩增出稳定可靠的条带。基于以上4条随机引物构建的聚类图能较好的从属水平区分各海龙样品,且与样品外观分析结果一致。基于LJ09,LJ19 2条引物的扩增结果构建的6种海龙的鉴别模式可以将海龙正品逐一鉴别出来。结论:随机引物扩增DNA技术能较好地鉴别海龙及其伪品。
Objective: To study the identification method of Syngnathus and its adulterants.Method: Random amplified polymorphic DNA(RAPD) was used to construct a dendrogram by UPGMA method based on Nei & Li's coefficient and a genetic affinity pattern for Syngnathus acus,Solenognathus hardwickii,Syngnathoides biaculeatus,Trachyrhamphus serratus,Halicampus koilomatodon,Microphis boaja.Result: Four primers,LJ04,LJ09,LJ16 and LJ19,from 18 random primers were used in the dendrogram which can differentiate Syngnathus in genus level and showed a great consistence with the appearance identification.The genetic affinity pattern based on primers LJ09 and LJ19 could be used to identify Syngnathus from its adulterants.Conclusion: RAPD is suitable to identify Syngnathus and its adulterants.

关键词(KeyWords): 海龙;伪品;RAPD
Syngnathus;adulterant;RAPD

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基金项目(Foundation): 国家自然科学基金项目(30672620)

作者(Author): 吴艳;刘佳;王梦月;鞠培俊;李晓波;
WU Yan,LIU Jia,WANG Mengyue,JU Peijun,LI Xiaobo(School of Pharmacy,Shanghai Jiao Tong University,Shanghai 200240,China)

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