王帅;郭巧生;史红专;芦鑫;陈盼盼;张会宁;严晓芦;
WANG Shuai;GUO Qiao-sheng;SHI Hong-zhuan;LU Xin;CHEN Pan-pan;ZHANG Hui-ning;YAN Xiao-lu;Institute of China Medicinal Materials,Nanjing Agricultural University;

• 1:南京农业大学中药材研究所

摘要(Abstract)：

为了探究短期添加雌二醇(17β-E2)对越冬蚂蟥生长、性腺发育及内在品质的影响,在蚂蟥越冬前,使养殖水体中17β-E2的质量浓度分别为0. 0,1. 0,10. 0,25. 0,50. 0,100. 0μg·L~(-1),养殖6周后停止添加,让蚂蟥自然冬眠,60 d出冬眠后测定蚂蟥生长指标、性腺指数、内源性类固醇激素及药材品质,石蜡切片观察精(卵)巢组织形态。结果表明,随着外源17β-E2浓度的升高,蚂蟥体质量、增重率、特定生长率、雌性性腺指数、卵母细胞发育以及内源雌激素水平呈先升高后降低的趋势,均高于对照组,其中17β-E2质量浓度为25μg·L~(-1)时蚂蟥体质量、增重率、特定生长率均显著高于其他各组(P<0. 05);当外源17β-E2质量浓度为50μg·L~(-1)时,卵母细胞发育、内源雌激素水平显著高于其他各组(P<0. 05);当外源17β-E2浓度大于50μg·L~(-1)时,对蚂蟥的雄性性腺指数、精母细胞发育、内源雄激素、孕激素水平产生显著抑制作用(P<0. 05);各组蚂蟥内源皮质激素水平无明显差异。综上,短期添加10～25μg·L~(-1)外源17β-E2,在不抑制雄性性腺发育的同时能促进越冬蚂蟥生长、卵细胞发育、提高抗凝血酶活性。
The present study was conducted to explores the effects of short-term addition of 17β-E2 on the growth,gonad development and internal quality of overwintering Whitmania pigra. Before overwintering,0. 0,1. 0,10. 0,25. 0,50. 0,100. 0 μg·L~(-1) of 17β-E2 were added to the aquaculture water for 6 weeks and then hibernated for 60 days. The changes of growth performance,gonad index,morphological structure of spermary( ovary),endogenous steroid hormones level and internal quality were measured. The results showed that the body weight,weight gain rate,specific growth rate,female gonad index,oocyte development and endogenous estrogen level of the leech increased first and then decreased with the increase of the concentration of exogenous 17β-E2,which were higher than those of the control group. The body weight,weight gain rate and specific growth rate of the leech at the concentration of 25 μg·L~(-1)17β-E2 were significantly higher than those of the other groups( P<0. 05),oocyte development and endogenous estrogen levels were significantly higher than those of other groups at the concentration of 50 μg·L~(-1)( P<0. 05). When the concentration of exogenous 17β-E2 was higher than 50 μg·L~(-1),the levels of male gonad index,spermatocyte development,endogenous androgen and progesterone were significantly inhibited( P< 0. 05). There was no significant difference in endogenous corticosteroid levels among the groups. In conclusion,short-term addition of exogenous 17β-E2 of 10-25 μg·L~(-1) could promote the growth of overwintering leeches,oocyte development and antithrombin activity without inhibiting the development of male gonads.

关键词(KeyWords)： 水蛭;17β-E2;性腺发育;冬眠;生长
Whitmania pigra;17β-E2;gonadal development;hibernation;growth

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(81673538,81473306);; 江苏现代农业产业技术体系建设专项(JATS[2018]283)

作者(Author): 王帅;郭巧生;史红专;芦鑫;陈盼盼;张会宁;严晓芦;
WANG Shuai;GUO Qiao-sheng;SHI Hong-zhuan;LU Xin;CHEN Pan-pan;ZHANG Hui-ning;YAN Xiao-lu;Institute of China Medicinal Materials,Nanjing Agricultural University;

Email:

DOI:

参考文献(References)：

• [1]中国药典.一部[S].2015:83.
• [2]史红专,刘飞,郭巧生.宽体金线蛭耗氧率与窒息点的初步研究[J].中国中药杂志,2005,30(23):1817.
• [3]史红专,郭巧生,朱再标,等.不同温度和pH对蚂蟥消化道酶活性影响的初步研究[J].中国中药杂志,2012,37(17):2538.
• [4]史红专,刘宏,郭巧生,等.不同月龄蚂蟥消化酶活性的研究[J].中国中药杂志,2015,40(14):2796.
• [5]李蒙蒙,郭巧生,史红专,等.不同养殖密度和换水频率对蚂蟥生长和内在品质影响的研究[J].中国中药杂志,2016,41(6):995.
• [6] Wang J,Shi H,Guo Q,et al. The impact of hibernation and arousal on energy metabolism and antioxidant defenses in leech(Whitmania pigra)[J]. Aquac Res,2018,49(1):188.
• [7] Liu F,Guo Q S,Shi H Z,et al. Genetic diversity and phylogenetic relationships among and within populations of Whitmania pigra and Hirudo nipponica based on ISSR and SRAP markers[J]. Biochem Syst Ecol,2013,51:215.
• [8]史红专,刘飞,郭巧生.温度对蚂蟥生长及摄食规律影响的初步研究[J].中国中药杂志,2006,31(23):1944.
• [9]闫士猛,史红专,郭巧生,等.蚂蟥冬眠临界温度的研究[J].中国中药杂志,2016,41(19):3615.
• [10]史红专,郭巧生,陆树松,等.不同月龄蚂蟥内在品质及最佳采收期研究[J].中国中药杂志,2009,34(23):3060.
• [11] Heald A,Selby P L,White A,et al. Progestins abrogate estrogen-induced changes in the insulin-like growth factor axis[J].Am J Obstet Gynecol,2000,183(3):593.
• [12]李松,何治德,钟其渝,等.17β-E2对大鳞副泥鳅生长和性腺分化的影响[J].淡水渔业,2015,45(1):83.
• [13]史红专,刘宏,郭巧生,等.蚂蟥性腺及生殖细胞周年发育的组织学研究[J].中国中药杂志,2018,43(2):299.
• [14]李霞,宋贞坪,王福景,等.雌激素对双齿围沙蚕性比、生长和卵母细胞发育的影响[J].大连海洋大学学报,2011,26(2):97.
• [15]王念民,张颖,曲秋芝,等.短期投喂雌二醇对施氏鲟幼鱼生长的影响[J].水产学杂志,2005(2):11.
• [16]吕德亮,李敏,吴反修,等.雌二醇对中间球海胆生长、性腺发育及胆固醇代谢的影响[J].大连海洋大学学报,2017,32(5):501.
• [17]沈蓓杰,杨筱珍,吴旭干,等.外源17β-E2对中华绒螯蟹Ⅲ~Ⅳ期卵巢发育以及内源雌激素水平的影响[J].上海海洋大学学报,2010,19(3):289.
• [18]张颖,孙大江,曲秋芝,等.不同温度下雌激素对施氏鲟早期性腺发育及血清性激素的影响[J].东北农业大学学报,2011,42(12):118.
• [19]宋海霞,翁幼竹,方永强.17β-雌二醇对鲻早期精子发生的影响及其作用机制[J].中国水产科学,2009,16(5):712.
• [20]张泉,周新华,张孝斌,等.苯甲酸雌二醇对大鼠睾丸组织发育的影响[J].中华男科学杂志,2005(7):490.
• [21]姚德冬,张晓雁,罗静波,等.外源性17-β雌二醇对养殖中华鲟血液若干生化指标的影响[J].淡水渔业,2016,46(5):14.
• [22]史红专,陈盼盼,郭巧生,等.蚂蟥性腺发育周期内性类固醇激素变化初步研究[J].中国中药杂志,2018,43(18):3639.
• [23] Rothbard S,Zohar Y,Zmora N,et al. Clearance of 17α-ethynyltestosterone from muscle of sex-inversed tilapia hybrids treated for growth enhancement with two doses of the androgen[J]. Aquaculture,1990,89(3):365.
• [24] Piferrer F. Endocrine sex control strategies for the feminization of teleost fish[J]. Aquaculture,2001,197(1):229.
• [25] Johnstone R,Macintosh D J,Wright R S.Elimination of orally administered 17α-methyltestosterone by Oreochromis mossambicus(tilapia)and Salmo gairdneri(rainbow trout)juveniles[J].Aquaculture,1983,35:249.