中国中药杂志

2019, v.44(15) 3239-3245

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短期添加17β-E2对越冬蚂蟥生长和性腺发育的影响
Effects of 17β-E2 on growth and gonad development of overwintering Whitmania pigra

王帅;郭巧生;史红专;芦鑫;陈盼盼;张会宁;严晓芦;
WANG Shuai;GUO Qiao-sheng;SHI Hong-zhuan;LU Xin;CHEN Pan-pan;ZHANG Hui-ning;YAN Xiao-lu;Institute of China Medicinal Materials,Nanjing Agricultural University;

摘要(Abstract):

为了探究短期添加雌二醇(17β-E2)对越冬蚂蟥生长、性腺发育及内在品质的影响,在蚂蟥越冬前,使养殖水体中17β-E2的质量浓度分别为0. 0,1. 0,10. 0,25. 0,50. 0,100. 0μg·L~(-1),养殖6周后停止添加,让蚂蟥自然冬眠,60 d出冬眠后测定蚂蟥生长指标、性腺指数、内源性类固醇激素及药材品质,石蜡切片观察精(卵)巢组织形态。结果表明,随着外源17β-E2浓度的升高,蚂蟥体质量、增重率、特定生长率、雌性性腺指数、卵母细胞发育以及内源雌激素水平呈先升高后降低的趋势,均高于对照组,其中17β-E2质量浓度为25μg·L~(-1)时蚂蟥体质量、增重率、特定生长率均显著高于其他各组(P<0. 05);当外源17β-E2质量浓度为50μg·L~(-1)时,卵母细胞发育、内源雌激素水平显著高于其他各组(P<0. 05);当外源17β-E2浓度大于50μg·L~(-1)时,对蚂蟥的雄性性腺指数、精母细胞发育、内源雄激素、孕激素水平产生显著抑制作用(P<0. 05);各组蚂蟥内源皮质激素水平无明显差异。综上,短期添加10~25μg·L~(-1)外源17β-E2,在不抑制雄性性腺发育的同时能促进越冬蚂蟥生长、卵细胞发育、提高抗凝血酶活性。
The present study was conducted to explores the effects of short-term addition of 17β-E2 on the growth,gonad development and internal quality of overwintering Whitmania pigra. Before overwintering,0. 0,1. 0,10. 0,25. 0,50. 0,100. 0 μg·L~(-1) of 17β-E2 were added to the aquaculture water for 6 weeks and then hibernated for 60 days. The changes of growth performance,gonad index,morphological structure of spermary( ovary),endogenous steroid hormones level and internal quality were measured. The results showed that the body weight,weight gain rate,specific growth rate,female gonad index,oocyte development and endogenous estrogen level of the leech increased first and then decreased with the increase of the concentration of exogenous 17β-E2,which were higher than those of the control group. The body weight,weight gain rate and specific growth rate of the leech at the concentration of 25 μg·L~(-1)17β-E2 were significantly higher than those of the other groups( P<0. 05),oocyte development and endogenous estrogen levels were significantly higher than those of other groups at the concentration of 50 μg·L~(-1)( P<0. 05). When the concentration of exogenous 17β-E2 was higher than 50 μg·L~(-1),the levels of male gonad index,spermatocyte development,endogenous androgen and progesterone were significantly inhibited( P< 0. 05). There was no significant difference in endogenous corticosteroid levels among the groups. In conclusion,short-term addition of exogenous 17β-E2 of 10-25 μg·L~(-1) could promote the growth of overwintering leeches,oocyte development and antithrombin activity without inhibiting the development of male gonads.

关键词(KeyWords): 水蛭;17β-E2;性腺发育;冬眠;生长
Whitmania pigra;17β-E2;gonadal development;hibernation;growth

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(81673538,81473306);; 江苏现代农业产业技术体系建设专项(JATS[2018]283)

作者(Author): 王帅;郭巧生;史红专;芦鑫;陈盼盼;张会宁;严晓芦;
WANG Shuai;GUO Qiao-sheng;SHI Hong-zhuan;LU Xin;CHEN Pan-pan;ZHANG Hui-ning;YAN Xiao-lu;Institute of China Medicinal Materials,Nanjing Agricultural University;

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