曹虹虹;严维花;郭爽;郝敏;张科卫;陆兔林;毛春芹;李林;宋嬿;
CAO Hong-hong;YAN Wei-hua;GUO Shuang;HAO Min;ZHANG Ke-wei;LU Tu-lin;MAO Chun-qin;LI Lin;SONG Yan;Nanjing University of Chinese Medicine;Shanghai Huayu Pharmaceutical Co.,Ltd.;

• 1:南京中医药大学
• 2:上海华宇药业有限公司

摘要(Abstract)：

建立超高效液相色谱法测定栀子中7种成分的方法,同时构建栀子指纹图谱识别模式,并结合多元统计分析评价不同产地栀子质量。采用Waters Acquity UPLC BEH C18色谱柱(2. 1 mm×50 mm,1. 7μm),以0. 1%甲酸水-乙腈为流动相,流速0. 4 m L·min-1,梯度洗脱,多波长检测进行含量测定和指纹图谱研究。含量测定结果显示各产地栀子7种成分总含量为浙江>福建>江西>四川。采用"中药色谱指纹图谱相似度评价系统"(2012版)进行相似度评价,样品的相似度均大于0. 95。建立了32批栀子药材共有图谱并标定了19个共有峰,经聚类分析(HCA)和主成分分析(PCA),将不同产地栀子样品分成3组,并进一步通过偏最小二乘法-判别分析(PLS-DA)筛选出栀子苷、西红花苷Ⅰ等5个差异成分。该研究所建立的栀子UPLC多成分含量测定和指纹图谱的方法准确可行,可作为不同产地栀子质量评价的一种手段。
An analysis method was established by UPLC fingerprint and then applied to simultaneous determination of multiple compounds in Gardeniae Fructus from different areas in China. Samples were separated on a Waters Acquity UPLC BEH C_(18)( 2. 1 mm×50 mm,1. 7 μm) column with 0. 1% formic acid-water and acetonitrile solution as gradient mobile phase at a flow rate of 0. 4 m L·min-1 at various wavelengths. The similarity of samples was over 0. 95 with ″Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine( 2012 edition) ″. The UPLC common fingerprints for 32 batches were established with 19 common peaks identified. The samples were divided into 3 groups analyzed by HCA and PCA. Five components were identified as the main compositions which caused the differences of chemical constituents in the samples from different areas with partial least squares discriminant analysis( PLS-DA). The content of the total components in each area was Zhejiang > Fujian > Jiangxi > Sichuan. This method was accurate and viable,could be used to evaluate the quality of Gardeniae Fructus.

关键词(KeyWords)： 栀子;产地;多成分含量测定;指纹图谱;多元统计分析
Gardeniae Fructus;region;determination of multi-component content;fingerprint;multivariate statistical analysis

Abstract:

Keywords:

基金项目(Foundation): 国家中药标准化项目(ZYY-2017-155);; 国家重点研发计划项目(2017YFC1701900)

作者(Author): 曹虹虹;严维花;郭爽;郝敏;张科卫;陆兔林;毛春芹;李林;宋嬿;
CAO Hong-hong;YAN Wei-hua;GUO Shuang;HAO Min;ZHANG Ke-wei;LU Tu-lin;MAO Chun-qin;LI Lin;SONG Yan;Nanjing University of Chinese Medicine;Shanghai Huayu Pharmaceutical Co.,Ltd.;

Email:

DOI: 10.19540/j.cnki.cjcmm.20190521.201

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