中国中药杂志

2009, v.34(22) 2903-2906

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RP-HPLC同时测定天麻中4种成分的含量
Simultaneous determination of four components in Gastrodia elata by RP-HPLC

闫宝庆;张晖芬;逄楠楠;陈晓辉;于治国;毕开顺;
YAN Baoqing,ZHANG Huifen,PANG Nannan,CHEN Xiaohui,YU Zhiguo,BI Kaishun(School of Pharmacy,Shenyang Pharmaceutical University,Shenyang 110016,China)

摘要(Abstract):

目的:建立RP-HPLC同时测定天麻药材中天麻素、腺苷、对羟基苯甲醇和对羟基苯甲醛的含量。方法:采用Kromasil C18色谱柱(4.6 mm×250 mm,5μm),以甲醇-0.1%醋酸水溶液为流动相,梯度洗脱,流速1.0 mL.min-1,检测波长270 nm,柱温35℃。结果:天麻素、腺苷、对羟基苯甲醇和对羟基苯甲醛分别在19.1~383(r=0.999 9),0.620~12.4(r=0.999 9),2.45~49.0(r=0.999 9),0.280~5.63 mg.L-1(r=0.999 6)与峰面积线性关系良好;平均加样回收率(n=9)均在96.7%~97.7%,RSD均小于1.6%。结论:本法准确可靠,分离度好,适用于天麻药材中天麻素、腺苷、对羟基苯甲醇和对羟基苯甲醛的含量测定。
Objective:To develop a reversed-phase HPLC method for simultaneous determination of gastrodin,adenosin,4-hydroxybenzyl alcohol and 4-hydroxybenzaldehyde in Rhizoma Gastrodia.Method:A Kromasil C18 column(4.6 mm×250 mm,5 μm) was used with a methanol-water-0.1% acetic acid gradient elution system.The eluates were detected at 270 nm,the flow rate was 1.0 mL·min-1 and the column temperature was 35 ℃.Result:The linear range of gastrodin,adenosin,4-hydroxybenzyl alcohol and 4-hydroxybenzaldehyde were 19.1-383(r=0.999 9),0.620-12.4(r=0.999 9),2.45-49.0(r=0.999 9),0.280-5.63 mg·L-1(r=0.999 6),respectively.The average recoveries(n=9) of the four components were 96.7%-97.7%,RSD<1.6%.Conclusion:The method is accurate,sensitive and reliable for determination of gastrodin,adenosin,4-hydroxybenzyl alcohol and 4-hydroxybenzaldehyde in Rhizoma Gastrodia.

关键词(KeyWords): 天麻;RP-HPLC;同时含量测定
Gastrodia elata;RP-HPLC;simultaneous quantification

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作者(Author): 闫宝庆;张晖芬;逄楠楠;陈晓辉;于治国;毕开顺;
YAN Baoqing,ZHANG Huifen,PANG Nannan,CHEN Xiaohui,YU Zhiguo,BI Kaishun(School of Pharmacy,Shenyang Pharmaceutical University,Shenyang 110016,China)

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