中国中药杂志

2020, v.45(19) 4699-4704

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SIRT1在人参皂苷Rg_1延缓D-gal诱导的卵巢早衰小鼠模型卵巢早衰中的作用
Effect of SIRT1 on delay of D-gal-induced premature ovarian failure in mice with ginsenoside Rg_1

刘小虎;赵志慧;周玥;王翠丽;韩艳军;周雯;
LIU Xiao-hu;ZHAO Zhi-hui;ZHOU Yue;WANG Cui-li;HAN Yan-jun;ZHOU Wen;Department of Histology and Embryology, School of Basic Medical Sciences, Dali University;

摘要(Abstract):

探索沉默信息调节因子2相关酶1(slient mating type information regulation 2 homolog 1,SIRT1)在人参皂苷Rg_1(ginsenoside Rg_1,Rg_1)延缓D-半乳糖(D-galactose,D-gal)诱导的卵巢早衰小鼠模型卵巢早衰中的作用。将SPF级雌性BALB/c小鼠54只随机分成PBS组、D-gal组、Rg_1组。D-gal组给予颈背部皮下注射D-半乳糖200 mg·kg~(-1)·d~(-1),连续给药42 d;PBS组给予颈背部注射等时等量磷酸盐缓冲液(phosphate buffered saline,PBS),连续注射42 d;Rg_1组在D-gal组处理基础上,于第15天开始给予腹腔注射Rg_1 20 mg·kg~(-1)·d~(-1),连续给药28 d,同时D-gal组和PBS组也在第15天开始给予腹腔注射等时等量PBS,连续给药28 d;给药后,检测小鼠体质量变化和卵巢质量系数改变,检测外周血雌二醇(estradiol 2,E_2)、黄体生成素(luteinizing hormone,LH)、超氧化物歧化酶(superoxide dismutase,SOD)、过氧化氢酶(catalase,CAT)、卵泡刺激素(follicle-stimulating hormone,FSH)水平,HE染色法检测卵巢形态学改变,荧光定量PCR和Western blot法检测衰老调控因子SIRT1表达的改变。结果显示,与PBS组比较,D-gal组小鼠体质量增长率明显减缓,卵巢质量系数下降;血清E_2,LH,SOD,CAT水平明显降低,FSH水平明显升高。Rg_1作用后,Rg_1组体质量增长率,卵巢质量系数以及血清E_2,LH,SOD,CAT水平均高于D-gal组,FSH水平低于D-gal组。HE染色检测显示PBS组卵巢卵泡形态结构正常;D-gal组卵泡数量减少,黄体空泡化明显,闭锁卵泡数量增多;与D-gal组比较,Rg_1组卵泡数量增多,黄体数量减少。与PBS组比较,D-gal组SIRT1表达下调;与D-gal组比较,Rg_1组SIRT1表达上调。研究结果提示,Rg_1具有延缓D-gal诱导的卵巢早衰小鼠模型中卵巢早衰的作用,衰老调控因子SIRT1在其中发挥重要功能。
To explore the effect of slient mating type information regulation 2 homolog 1(SIRT1) on the delay of D-galactose(D-gal) induced premature ovarian failure in mice with ginsenoside Rg_1(Rg_1). Fifty-four female SPF BALB/c mice were randomly divided into PBS group, D-gal group, and Rg_1 group. In the D-gal group, D-galactose(200 mg·kg~(-1)·d~(-1)) was injected subcutaneously into the neck and back for 42 days. In the PBS group, the equal amount of phosphate buffered saline(PBS) was injected into the neck and back for 42 days. In addition to the therapy of D-gal group, Rg_1 group was given Rg_1(20 mg·kg~(-1)·d~(-1)) through the intraperitoneal injection on the 15 th day for 28 days. At the same time, the D-gal group and the PBS group were also given an equal amount of PBS through hintraperitoneal injection on the 15 th day for 28 days. The changes in body weight and ovarian weight coefficient of mice were detected. Expressions of estradiol 2(E_2), luteinizing hormone(LH), superoxide dismutase(SOD), catalase(CAT) and follicle-stimulating hormone(FSH) in peripheral blood were detected. Morphological changes of ovaries were detected by HE staining. Changes in expression of aging regulator SIRT1 were detected by fluorescent quantitative PCR and Western blot. The results showed that compared with the PBS group, the body weight growth rate of the D-gal group was significantly slowed, the ovarian weight coefficient was decreased, the serum levels of E_2, LH, SOD, CAT were significantly reduced, and FSH was significantly increased. After the administration with Rg_1, the body weight growth rate, ovarian weight coefficient, serum levels of E_2, LH, SOD, and CAT in the mice were higher than those in the D-gal group, while FSH was lower than those in the D-gal group. HE staining showed that the follicular morphology and structure of the PBS group were normal; the number of follicles in the D-gal group was reduced, the corpus luteum was vacuolated, and the number of atretic follicles was increased. Compared with the D-gal group, the number of follicles in the Rg_1 group was increased, whereas the number of corpus luteum was decreased. Compared with the PBS group, SIRT1 expression was down-regulated in the D-gal group, while SIRT1 expression was up-regulated in the Rg_1 group compared with the D-gal group. The results suggested that Rg_1 could delay D-gal-induced premature ovarian failure in a mouse model of premature ovarian failure, and SIRT1 played an important role in this.

关键词(KeyWords): 人参皂苷Rg_1;卵巢早衰;SIRT1;D-半乳糖
ginsenoside Rg_1;premature ovarian failure;SIRT1;D-galactose(D-gal)

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(81860038,81660731,81873103);; 云南省教育厅科学研究基金项目(2020Y0541)

作者(Author): 刘小虎;赵志慧;周玥;王翠丽;韩艳军;周雯;
LIU Xiao-hu;ZHAO Zhi-hui;ZHOU Yue;WANG Cui-li;HAN Yan-jun;ZHOU Wen;Department of Histology and Embryology, School of Basic Medical Sciences, Dali University;

Email:

DOI: 10.19540/j.cnki.cjcmm.20200427.403

参考文献(References):

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