中国中药杂志

2004, (08)

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高效液相色谱法测定飞龙掌血中异茴芹香豆素含量
Quantification of isopimpinellin in root of Toddalia Asiatica by HPLC

郝小燕,曹晓红,梁妍,沈月毛
HAO Xiao-yan~1, CAO Xiao-hong~2, LIANG Yan~1, SHEN Yue-mao~2(1.Department of Pharmacy Guiyang Medical college, Guiyang 550004, China;2.The Key Laboratory of Chemistry for Natural Products of Guizhou Province and Chinese Academy of Sciences, Guiyang 550002, China)

摘要(Abstract):

目的 :用高效液相色谱法对苗药飞龙掌血中异茴芹香豆素含量进行测定。方法 :采用HypersilC18柱为分析柱 ,以甲醇 水 (70∶30 )为流动相 ,检测波长 30 6nm。结果 :线性范围 0 .0 0 4 2 0~ 0 .4 2 0 μg ,平均回收率 99.7% ,RSD =2 .8%。结论 :本法简便、准确、重现性好 ,可用于飞龙掌血中异茴芹香豆素的含量测定
Objective: To Determine the content of isopimpinellin in root of Toddalia asiatica. Methods: A HPLC method was set up. Using Hypersil C_(18) column and methanol-water (70∶30) as mobile phase, with the detection wavelength at 306 nm. Result: The linear range of isopimpinellin was 0.004 20~0.420 μg. The average recovery was 99.7% and the RSD 2.8%. Conclusion: The method is simple and accurate, with good reproducibility, and can be used as a quantitative analysis method for isopimpinellin.

关键词(KeyWords): 飞龙掌血;异茴芹香豆素;高效液相色谱法;含量测定
Toddalia asiatica; isopimpinellin; HPLC; content determination

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作者(Author): 郝小燕,曹晓红,梁妍,沈月毛
HAO Xiao-yan~1, CAO Xiao-hong~2, LIANG Yan~1, SHEN Yue-mao~2(1.Department of Pharmacy Guiyang Medical college, Guiyang 550004, China;2.The Key Laboratory of Chemistry for Natural Products of Guizhou Province and Chinese Academy of Sciences, Guiyang 550002, China)

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