中国中药杂志

2017, v.42(11) 2123-2130

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基于UPLC-Q-TOF-MS/MS技术的短管兔耳草化学成分快速识别研究
Fast identification of constituents of Lagotis brevituba by using UPLC-Q-TOF-MS/MS method

谢晶;张丽;曾金祥;李敏;王娟;谢雄雄;钟国跃;罗光明;袁金斌;梁健;
XIE Jing;ZHANG Li;ZENG Jin-xiang;LI Min;WANG Juan;XIE Xiong-xiong;ZHONG Guo-yue;LUO Guang-ming;YUAN Jin-bin;LIANG Jian;Research Center of Traditional Chinese Medicine Resources and Minority Medicine,Jiangxi University of Traditional Chinese Medicine;Department of Budget and Finance,Jiangxi University of Traditional Chinese Medicine;

摘要(Abstract):

采用超高效液相色谱-串联四极杆飞行时间质谱(UPLC-Q-TOF/MS)技术快速分析鉴别短管兔耳草的化学成分,为其临床应用提供参考依据,试验采用UPLC-Q-TOF-MS/MS仪器,YMC-Triart C_(18)色谱柱(2.1 mm×100 mm,1.9μm),以乙腈-0.2%甲酸水溶液为流动相梯度洗脱;质谱采用电喷雾(ESI)离子源,在负离子模式下采集数据,通过保留时间、精确分子离子峰和二级质谱裂解碎片,并结合参考文献,对短管兔耳草成分进行鉴定,该实验共鉴别出22个化合物,其中黄酮类化合物11个,苯乙醇苷类化合物6个,环烯醚萜类化合物1个,有机酸类化合物4个。UPLC-Q-TOF-MS/MS方法能快速鉴别短管兔耳草中的各类化学成分,方法简单,快速,可为短管兔耳草的临床应用提供物质依据。
The chemical constituents of Lagotis brevituba were rapidly determined and analyzed by using ultra performance liquid chromatography tandem quadrupole time of flight mass spectrometry( UPLC-Q-TOF-MS/MS) method,providing material basis for the clinical application of L. brevituba. The separation was performed on UPLC YMC-Triart C_(18)(2. 1 mm × 100 mm,1. 9 μm) column,with acetonitrile-water containing 0. 2% formic acid as mobile phase for gradient elution. The flow rate was 0. 4 mL ·min~(-1)gradient elution and column temperature was 40 ℃,the injection volume was 2 μL. ESI ion source was used to ensure the data collected in a negative ion mode. The chemical components of L. brevituba were identified through retention time,exact relative molecular mass,cleavage fragments of MS/MS and reported data. The results showed that a total of 22 compounds were identified,including 11 flavones,6 phenylethanoid glycosides,1 iridoid glucosides,and 4 organic acid. The UPLC-Q-TOF-MS/MS method could fast identify the chemical components of L. brevituba,providing valuable information about L. brevituba for its clinical application.

关键词(KeyWords): 短管兔耳草;UPLC-Q-TOF-MS/MS;化学成分;快速鉴别
Lagotis brevituba;UPLC-Q-TOF-MS/MS;chemical constituents;fast identification

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(81660648)

作者(Author): 谢晶;张丽;曾金祥;李敏;王娟;谢雄雄;钟国跃;罗光明;袁金斌;梁健;
XIE Jing;ZHANG Li;ZENG Jin-xiang;LI Min;WANG Juan;XIE Xiong-xiong;ZHONG Guo-yue;LUO Guang-ming;YUAN Jin-bin;LIANG Jian;Research Center of Traditional Chinese Medicine Resources and Minority Medicine,Jiangxi University of Traditional Chinese Medicine;Department of Budget and Finance,Jiangxi University of Traditional Chinese Medicine;

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DOI: 10.19540/j.cnki.cjcmm.20170307.012

参考文献(References):

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