中国中药杂志

2006, (13) 1059-1062

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复方大青叶注射液HPLC指纹图谱研究
Fingerprint analysis of composite Folii Isatidis injection by HPLC

刘瑞;刘志刚;李磊;任冲;袁波;李发美;
LIU Rui,LIU Zhi-gang,LI Lei,REN Chong,YUAN Bo,LI Fa-mei(School of Pharmacy,Shenyang Pharmaceutical University,Shenyang 110016,China)

摘要(Abstract):

目的:采用HPLC建立复方大青叶注射液的指纹图谱测定方法。方法:采用Diamonsil C18色谱柱,甲醇-水-磷酸为流动相进行梯度洗脱,流速1.0 mL.min-1,检测波长254 nm。结果:建立了复方大青叶注射液的HPLC对照指纹图谱,标示了注射液中的20个共有峰,并初步确定注射液的相似度阈值为0.85。结论:该方法准确、重复性好,为复方大青叶注射液的质量控制提供了依据。
Objective: To develop the chromatographic fingerprints of composite Folii Isatidis injection by HPLC.Method: The separation was performed on a Diamonsil C_(18) column with a mobile phase consisting of methanol-water-phosphoric acid as gradient eluent at the flow rate of 1.0 mL·min~(-1).The UV detection was set at 254 nm.Result: A standard HPLC fingerprint procedure was developed for composite Folii Isatidis injection,with 20 common peaks and a similarity threshold of 9.0 established.Conclusion: This method was accurate,repeatable and useful for the quality control of composite Folii Isatidis injection.

关键词(KeyWords): 复方大青叶注射液;质量控制;指纹图谱;相似度
Composite Folii Isatidis injection;quality control;HPLC fingerprint;similarity

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基金项目(Foundation): 国家科技攻关计划项目(2001BA701A56)子项目

作者(Author): 刘瑞;刘志刚;李磊;任冲;袁波;李发美;
LIU Rui,LIU Zhi-gang,LI Lei,REN Chong,YUAN Bo,LI Fa-mei(School of Pharmacy,Shenyang Pharmaceutical University,Shenyang 110016,China)

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